Change search
ReferencesLink to record
Permanent link

Direct link
Asymptomatic Helminth Infection in Active Tuberculosis Is Associated with Increased Regulatory and Th-2 Responses and a Lower Sputum Smear Positivity
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Medicine and Health Sciences. University of Gondar, Ethiopia.
University of Addis Ababa, Ethiopia.
Vastervik Hospital, Sweden.
University of Gondar, Ethiopia.
Show others and affiliations
2015 (English)In: PLoS Neglected Tropical Diseases, ISSN 1935-2727, E-ISSN 1935-2735, Vol. 9, no 8, e0003994Article in journal (Refereed) Published
Abstract [en]

Background The impact of intestinal helminth infection on the clinical presentation and immune response during active tuberculosis (TB) infection is not well characterized. Our aim was to investigate whether asymptomatic intestinal helminth infection alters the clinical signs and symptoms as well as the cell mediated immune responses in patients with active TB.

Methodology Consecutive, newly diagnosed TB patients and healthy community controls (CCs) were recruited in North-west Ethiopia. TB-score, body mass index and stool samples were analyzed. Cells from HIV-negative TB patients (HIV-/TB) and from CCs were analyzed for regulatory T-cells (Tregs) and cytokine responses using flow cytometry and ELISPOT, respectively.

Results A significantly higher ratio of helminth co-infection was observed in TB patients without HIV (Helm+/HIV-/TB) compared to HIV negative CCs, (40% (121/306) versus 28% (85/306), p = 0.003). Helm+/HIV-/TB patients showed significantly increased IL-5 secreting cells compared to Helm-/HIV-/TB (37 SFU (IQR:13-103) versus 2 SFU (1-50); p = 0.02, n = 30). Likewise, levels of absolute Tregs (9.4 (3.2-16.7) cells/mu l versus 2.4 (1.1-4.0) cells/mu l; p = 0.041) and IL-10 secreting cells (65 SFU (7-196) versus 1 SFU (0-31); p = 0.014) were significantly higher in Helm+/HIV-/TB patients compared to Helm-/HIV-/TB patients. In a multivariate analysis, a lower rate of sputum smear positivity for acid fast bacilli, lower body temperature, and eosinophilia were independently associated with helminth infection in TB patients.

Conclusions Asymptomatic helminth infection is associated with increased regulatory T-cell and Th2-type responses and a lower rate of sputum smear positivity. Further studies are warranted to investigate the clinical and immunological impact of helminth infection in TB patients.

Place, publisher, year, edition, pages
PUBLIC LIBRARY SCIENCE , 2015. Vol. 9, no 8, e0003994
National Category
Microbiology in the medical area
URN: urn:nbn:se:liu:diva-121763DOI: 10.1371/journal.pntd.0003994ISI: 000360708200058PubMedID: 26248316OAI: diva2:859348

Funding Agencies|SAREC/SIDA; EU/EDCTP [JP 10800.006]; Swedish Research Council; Swedish Heart and Lung Foundation (Oscar II Jubilee Foundation); Wallenberg Foundation; Armauer Hansen Research Institute

Available from: 2015-10-06 Created: 2015-10-05 Last updated: 2015-10-27

Open Access in DiVA

fulltext(638 kB)65 downloads
File information
File name FULLTEXT01.pdfFile size 638 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Abate, EbbaStendahl, OlleSchön, Thomas
By organisation
Division of Microbiology and Molecular MedicineFaculty of Medicine and Health Sciences
In the same journal
PLoS Neglected Tropical Diseases
Microbiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar
Total: 65 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 210 hits
ReferencesLink to record
Permanent link

Direct link