Comparative genome analysis of Lysinibacillus B1-CDA, a bacterium that accumulates arsenics
2015 (English)In: Genomics, ISSN 0888-7543, E-ISSN 1089-8646, Vol. 106, no 6, 384-392 p.Article in journal (Refereed) Published
Previously, we reported an arsenic resistant bacterium Lysinibacillus sphaericus B1-CDA, isolated from an arsenic contaminated lands. Here, we have investigated its genetic composition and evolutionary history by using massively parallel sequencing and comparative analysis with other known Lysinibacillus genomes. Assembly of the sequencing reads revealed a genome of ~ 4.5 Mb in size encompassing ~ 80% of the chromosomal DNA. We found that the set of ordered contigs contains abundant regions of similarity with other Lysinibacillus genomes and clearly identifiable genome rearrangements. Furthermore, all genes of B1-CDA that were predicted be involved in its resistance to arsenic and/or other heavy metals were annotated. The presence of arsenic responsive genes was verified by PCR in vitro conditions. The findings of this study highlight the significance of this bacterium in removing arsenics and other toxic metals from the contaminated sources. The genetic mechanisms of the isolate could be used to cope with arsenic toxicity.
Place, publisher, year, edition, pages
Elsevier, 2015. Vol. 106, no 6, 384-392 p.
Toxic metals, Bioremediation, Genome sequencing, de novo assembly
Biochemistry and Molecular Biology Bioinformatics and Systems Biology
Research subject Natural sciences
IdentifiersURN: urn:nbn:se:his:diva-11575DOI: 10.1016/j.ygeno.2015.09.006ISI: 000365613100010PubMedID: 26387925ScopusID: 2-s2.0-84948102629OAI: oai:DiVA.org:his-11575DiVA: diva2:858341
FunderSida - Swedish International Development Cooperation Agency, AKT-2010-18