Change search
ReferencesLink to record
Permanent link

Direct link
Pathogenic Yersinia Promotes Its Survival by Creating an Acidic Fluid-Accessible Compartment on the Macrophage Surface
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Show others and affiliations
2015 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 10, no 8, e0133298Article in journal (Refereed) Published
Abstract [en]

Microbial pathogens and host immune cells each initiate events following their interaction in an attempt to drive the outcome to their respective advantage. Here we show that the bacterial pathogen Yersinia pseudotuberculosis sustains itself on the surface of a macrophage by forming acidic fluid-accessible compartments that are partially bounded by the host cell plasma membrane. These Yersinia-containing acidic compartments (YACs) are bereft of the early endosomal marker EEA1 and the lysosomal antigen LAMP1 and readily form on primary macrophages as well as macrophage-like cell lines. YAC formation requires the presence of the Yersinia virulence plasmid which encodes a type III secretion system. Unexpectedly, we found that the initial formation of YACs did not require translocation of the type III effectors into the host cell cytosol; however, the duration of YACs was markedly greater in infections using translocation-competent Y. pseudotuberculosis strains as well as strains expressing the effector YopJ. Furthermore, it was in this translocation- and YopJ-dependent phase of infection that the acidic environment was critical for Y. pseudotuberculosis survival during its interaction with macrophages. Our findings indicate that during its extracellular phase of infection Y. pseudotuberculosis initiates and then, by a separate mechanism, stabilizes the formation of a highly intricate structure on the surface of the macrophage that is disengaged from the endocytic pathway.

Place, publisher, year, edition, pages
2015. Vol. 10, no 8, e0133298
National Category
Cell and Molecular Biology
URN: urn:nbn:se:umu:diva-108136DOI: 10.1371/journal.pone.0133298ISI: 000359493600011OAI: diva2:855133
Available from: 2015-09-18 Created: 2015-09-04 Last updated: 2015-09-18Bibliographically approved

Open Access in DiVA

fulltext(7525 kB)93 downloads
File information
File name FULLTEXT01.pdfFile size 7525 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Westermark, LindaFällman, Maria
By organisation
Department of Molecular Biology (Faculty of Medicine)
In the same journal
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 93 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 159 hits
ReferencesLink to record
Permanent link

Direct link