Brusatol inhibits the response of cultured beta-cells to pro-inflammatory cytokines in vitro
2015 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 460, no 3, 868-872 p.Article in journal (Refereed) Published
Brusatol is a natural terpenoid that is capable of inducing a variety of biological effects. We presently report that this substance dramatically improves beta-cell survival when exposed to pro-inflammatory cytokines (IL-1 beta and IFN gamma) in vitro. This was observed in insulin producing rat (RIN-5AH), mouse (beta TC6) and human (EndoC-beta H1) beta-cell lines. Brusatol prevented beta-cell oxidative stress in response to cytokines and counteracted induction of iNOS on the protein level. Brusatol, however, block neither the cytokine-induced increase of iNOS mRNA, nor NF-kappa B activation, suggesting that inhibition of iNOS protein expression relies on posttranscriptional mechanism. This indicates that brusatol acts via a novel protective pathway, which may represent a more promising way of improving beta-cell function and survival.
Place, publisher, year, edition, pages
2015. Vol. 460, no 3, 868-872 p.
Brusatol, Cytokines, Beta-cells, Oxidative stress, iNOS
Cell and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-262449DOI: 10.1016/j.bbrc.2015.03.124ISI: 000359885300061PubMedID: 25824046OAI: oai:DiVA.org:uu-262449DiVA: diva2:853837
FunderSwedish Diabetes Association