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MS for investigation of time-dependent protein adsorption on surfaces in complex biological samples
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
2015 (English)In: Future Science OA, ISSN 2056-5623, 32Article in journal (Refereed) Published
Abstract [en]

Aim: This study aims at developing a nondestructive way for investigating proteinadsorption on surfaces such as biomaterials using mass spectrometry. Methods: Ventricular cerebrospinal fluid in contact with poly carbonate membranes were usedas adsorption templates and on-surface enzymatic digestion was applied to desorbproteins and cleave them into peptides. Mass spectrometric analysis provided bothprotein identification and determination of protein specific adsorption behavior. Results: In general, the adsorption increased with incubation time but also proteinspecifictime-resolved adsorption patterns from the complex protein solutionwere discovered. Conclusion: The method developed is a promising tool for thecharacterization of biofouling, which sometimes causes rejection and encapsulationof implants and can be used as complement to other surface analytical techniques.

One problem associated with artificial materials in the body is that proteins in thebody interact with the surface, which sometimes causes rejection of the implant.In this study, a method for investigating the time-dependent protein adsorptionon a surface originating from a complex biological protein solution was developed.Compared with other surface analyses, this method can identify what proteins thatadsorbs on the surface. In addition, determination of protein-specific adsorptionbehavior in relation to incubation was possible

Place, publisher, year, edition, pages
2015. 32
National Category
Chemical Sciences Engineering and Technology
Identifiers
URN: urn:nbn:se:uu:diva-262022DOI: 10.4155/fso.15.32OAI: oai:DiVA.org:uu-262022DiVA: diva2:851814
Funder
Åke Wiberg Foundation, M14–0127Magnus Bergvall Foundation, 2014-00367
Available from: 2015-09-07 Created: 2015-09-07 Last updated: 2017-01-24Bibliographically approved

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Publisher's full texthttp://dx.doi.org/10.4155/fso.15.32

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