Change search
ReferencesLink to record
Permanent link

Direct link
Design of an LC-MS/MS method for measuring concentrations of Cyclosporine A and Tacrolimus from dried blood spots
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Analytical Pharmaceutical Chemistry.
2015 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Patients that have undergone organ transplantation are life-long treated with immunosuppressant drugs and these have to be monitored regularly to get the desired effect of suppressing the immune system. To monitor the drug concentration normally a venous blood sample is collected at a clinic but the use of dried blood spots (DBS) as a matrix for drug monitoring for immunosuppressant drugs will make home sampling possible for this patient group. The aim of this study was to develop and validate a bioanalytical method for quantifying cyclosporine A and tacrolimus in dried blood spots. The method consist of punching out a 5 mm disc from a blood spot , followed by extracting the spot in a 96-well hydrophobic filter plate with 150 µL extraction solution containing internal standard (ascomycin and cyclosporine A d12) in a methanol water solution (80:20v/v%). The extract is then centrifuged through the filter plate down in a 96-deep well plate and injected on the LC-MS/MS, with an analysis time of 2.5min. The method will be validated in accordance with the guidelines set by the European Medicines Agency with additions specific to DBS. The method is not fully validated but will be in due time. The validated parameters show a robust and fast analysing method that has the prospects of being used for analysing DBS samples for patients and in the future can possibly be used by patients in home environment.

Place, publisher, year, edition, pages
2015. , 23 p.
UPTEC K, ISSN 1650-8297 ; 15027
Keyword [en]
LC-MS/MS, Dried blood spots, Immunosuppressive drugs
National Category
Analytical Chemistry
URN: urn:nbn:se:uu:diva-261083OAI: diva2:849944
External cooperation
Karolinska Universitetslaboratoriet
Subject / course
Educational program
Master Programme in Chemical Engineering
Available from: 2015-09-01 Created: 2015-08-29 Last updated: 2016-09-01Bibliographically approved

Open Access in DiVA

fulltext(742 kB)3 downloads
File information
File name FULLTEXT01.pdfFile size 742 kBChecksum SHA-512
Type fulltextMimetype application/pdf

By organisation
Analytical Pharmaceutical Chemistry
Analytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar
Total: 3 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 245 hits
ReferencesLink to record
Permanent link

Direct link