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Candidatus Neoehrlichia mikurensis in Ticks from Migrating Birds in Sweden
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology and Infectious Medicine.
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2015 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 10, no 7, e0133250Article in journal (Refereed) Published
Abstract [en]

Candidatus Neoehrlichia mikurensis (CNM; family Anaplasmataceae) was recently recognized as a potential tick-borne human pathogen. The presence of CNM in mammals, in host-seeking Ixodes ticks and in ticks attached to mammals and birds has been reported recently. We investigated the presence of CNM in ornithophagous ticks from migrating birds. A total of 1,150 ticks (582 nymphs, 548 larvae, 18 undetermined ticks and two adult females) collected from 5,365 birds captured in south-eastern Sweden was screened for CNM by molecular methods. The birds represented 65 different species, of which 35 species were infested with one or more ticks. Based on a combination of morphological and molecular species identification, the majority of the ticks were identified as Ixodes ricinus. Samples were initially screened by real-time PCR targeting the CNM 16S rRNA gene, and confirmed by a second real-time PCR targeting the groEL gene. For positive samples, a 1260 base pair fragment of the 16S rRNA gene was sequenced. Based upon bacterial gene sequence identification, 2.1% (24/1150) of the analysed samples were CNM-positive. Twenty-two out of 24 CNM-positive ticks were molecularly identified as I. ricinus nymphs, and the remaining two were identified as I. ricinus based on morphology. The overall CNM prevalence in I. ricinus nymphs was 4.2%. None of the 548 tested larvae was positive. CNM-positive ticks were collected from 10 different bird species. The highest CNM-prevalences were recorded in nymphs collected from common redpoll (Carduelis flammea, 3/7), thrush nightingale (Luscinia luscinia, 2/29) and dunnock (Prunella modularis, 1/17). The 16S rRNA sequences obtained in this study were all identical to each other and to three previously reported European strains, two of which were obtained from humans. It is concluded that ornithophagous ticks may be infected with CNM and that birds most likely can disperse CNM-infected ticks over large geographical areas.

Place, publisher, year, edition, pages
2015. Vol. 10, no 7, e0133250
National Category
Infectious Medicine Microbiology in the medical area
Identifiers
URN: urn:nbn:se:uu:diva-260844DOI: 10.1371/journal.pone.0133250ISI: 000358622000071OAI: oai:DiVA.org:uu-260844DiVA: diva2:848609
Funder
Carl Tryggers foundation Magnus Bergvall Foundation
Note

Funding for this study came from the Medical Research Council of Southeast Sweden (grant numbers FORSS-307591 and FORSS-387231), Carl Trygger's Foundation for Scientific Research, Langmanska kulturfonden, Magnus Bergvall's Foundation for Scientific Research, Helge Ax:son Johnson's Foundation and the EU Interreg IVA project ScandTick.

Available from: 2015-08-25 Created: 2015-08-25 Last updated: 2017-12-04Bibliographically approved

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Labbé, Lisa SandelinSalaneck, ErikJaenson, Thomas G. T.Olsen, Björn
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