Change search
ReferencesLink to record
Permanent link

Direct link
Effekter av lågdosbestrålning vid p53-medierad celldöd
KTH, School of Chemical Science and Engineering (CHE).
2015 (Swedish)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
Response of p53 mediated cell death to low dose irradiation (English)
Abstract [en]

The aim of the study was to investigate if PRIMA-1, a drug that reactivate the tumour suppressor gene p53, will have a synergistic effect when used in combination with γ-irradiation in a human small cell lung cancer cell line (U-1690), which show a low-dose hypersensitivity in clonogenic survival experiments. Clonogenic cell survival after incubation with PRIMA-1 alone in concentrations from 0.1 to 30 µM or in combination with γ-irradiation with a 137Cs source was performed. The standard LQ model, and the repairable-conditionally repairable damage model, the RCR model, were fitted to the experimental data. PRIMA-1 showed a time and concentration dependent cytotoxic, and 10 µM killed 60 % of cells during 72 hours incubation. The cells show low-dose hypersensitivity calculated with the RCR model. The combined treatment with 8 µM PRIMA-1 had a synergistic effect with irradiation doses from 2 Gy and above. The overall conclusion is that a combination with PRIMA-1 and irradiation is beneficial to achieve the best effect.

Place, publisher, year, edition, pages
2015. , 42 p.
Keyword [en]
PRIMA-1, 137Cs, p53, synergy, hypersensitivity
National Category
Other Physics Topics
URN: urn:nbn:se:kth:diva-172448OAI: diva2:848284
Available from: 2015-08-24 Created: 2015-08-24 Last updated: 2015-08-24Bibliographically approved

Open Access in DiVA

fulltext(1055 kB)36 downloads
File information
File name FULLTEXT01.pdfFile size 1055 kBChecksum SHA-512
Type fulltextMimetype application/pdf

By organisation
School of Chemical Science and Engineering (CHE)
Other Physics Topics

Search outside of DiVA

GoogleGoogle Scholar
Total: 36 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 55 hits
ReferencesLink to record
Permanent link

Direct link