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Metabolisk modellering av butanol produktion i cyanobakterie
KTH, School of Biotechnology (BIO).
2015 (Swedish)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
Flyx balance analysis of cyanobacteria metabolism for butanol production (English)
Abstract [en]

Engineering microorganisms at the systems level is recognized to be the future of metabolic engineering. Thanks to the development of genome annotation, mcroorganisms can be understood, as never before, and be reconstructed in the form of computational models. Flux balance analysis provides a deep insight intocellular metabolism and can guide metabolic engineering strategies. In particular, algorithms can assess the cellular complexity of the metabolism and hint at genetic interventions to improve product productivity. In this work, Synechosystis PCC6803 metabolism was invesetigated in silico. Genetic interventions could besuggested to couple butanol synthesis to growth as a way to improve currentproductivities. Cofactor recycling and, in particular, buffering mechanisms were shown to be important targets. Creating a cofacor imbalance and removing thesebuffering mechanisms is an important driving force. This forces a carbon flux through butanol synthesis to maintain cofactor balance and sustain growth.

Place, publisher, year, edition, pages
Keyword [en]
cyanobacteria, biofuel, metabolic modeling, flux balance analysis, genomics
National Category
Engineering and Technology
URN: urn:nbn:se:kth:diva-172095OAI: diva2:845671
Available from: 2015-09-08 Created: 2015-08-12 Last updated: 2015-09-08Bibliographically approved

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