Detection and quantitative estimation of spurious double stranded DNA formation during reverse transcription in bateria using tagRNA-seq
2015 (English)In: RNA Biology, ISSN 1547-6286, E-ISSN 1555-8584Article in journal (Refereed) Published
Standard RNA-seq has a well know tendency to generate "ghost" antisense reads due to formation of spurious second strand cDNA in the sequencing process. We recently reported on a novel variant of RNA-seq coined "tagRNA-seq" introduced for the purpose of distinguishing primary from processed transcripts in bacteria. Incidentally, the additional information provided by the tag is also very suitable for detection of true anti-sense RNA transcripts and quantification of spurious antisense signals in a sample. We briefly explain how to perform such a detection and illustrate on previously published datasets.
Place, publisher, year, edition, pages
Taylor & Francis, 2015.
tagRNA-seq, spurious second strand cDNA, antisense RNA, complementary DNA, transcriptome, transcript discovery
Bioinformatics and Systems Biology Microbiology
Research subject Biotechnology; Biological Physics
IdentifiersURN: urn:nbn:se:kth:diva-171378DOI: 10.1080/15476286.2015.1071010ISI: 000361473300018ScopusID: 2-s2.0-84949803433OAI: oai:DiVA.org:kth-171378DiVA: diva2:843538
QC 201508112015-07-292015-07-292015-10-20Bibliographically approved