Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp PCC 6803
2015 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 10, no 6, e0130904Article in journal (Refereed) Published
The biosynthesis pathway of carotenoids in cyanobacteria is partly described. However, the subcellular localization of individual steps is so far unknown. Carotenoid analysis of different membrane subfractions in Synechocystis sp. PCC6803 shows that "light" plasma membranes have a high carotenoid/protein ratio, when compared to "heavier" plasma membranes or thylakoids. The localization of CrtQ and CrtO, two well-defined carotenoid synthesis pathway enzymes in Synechocystis, was studied by epitope tagging and western blots. Both enzymes are locally more abundant in plasma membranes than in thylakoids, implying that the plasma membrane has higher synthesis rates of beta-carotene precursor molecules and echinenone.
Place, publisher, year, edition, pages
2015. Vol. 10, no 6, e0130904
IdentifiersURN: urn:nbn:se:umu:diva-106601DOI: 10.1371/journal.pone.0130904ISI: 000356567400164PubMedID: 26083372OAI: oai:DiVA.org:umu-106601DiVA: diva2:843322