Validation study: HemoCue Hb 201 + as a tool in comparative physiological field studies on avian blood
Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Haemoglobin concentration is becoming a widely popular parameter to use to assess physiological condition within a broad range of species. Assessments of large populations would preferable be done in field to receive quick results and avoid confounding factors associated with transport of blood. A validation study is here performed to see how well the point-of-care device HemoCue Hb 201 + can assess haemoglobin concentration on avian blood. Nucleated erythrocytes have previously been pointed out as something that makes it problematic to apply HemoCue Hb 201 +, designed for human blood, on avian blood. Here it is shown that HemoCue Hb 201 + accurately can estimate haemoglobin concentration for chicken-, tinamou-, and ostrich blood. However, manipulation of ostrich cells, to yield a larger mean corspuscular volume, results in HemoCue Hb 201 + overestimating haemoglobin concentration. A large mean corpuscular volume could therefore be something that impair accuracy in values retrieved with HemoCue Hb 201 +. This study shows that HemoCue Hb 201 + seems possible to apply on avian blood to some extent, but highlights the importance of validation studies when applying this device on new species.
Place, publisher, year, edition, pages
2015. , 19 p.
HemoCue Hb 201 +, Drabkin´s method, validation study, avian blood, haemoglobin concentration, mean corpuscular volume
Other Biological Topics
IdentifiersURN: urn:nbn:se:liu:diva-120106ISRN: LiTH-IFM- Ex--15/3039--SEOAI: oai:DiVA.org:liu-120106DiVA: diva2:840616
Subject / course