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Hur påverkas CellaVision DM1200:s förklassificering av leukocyternas infärgning?
2015 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [sv]

Sammansättningen av leukocyter i perifert blod varierar med olika sjukdomstillstånd och analys av cellerna är avgörande vid diagnostisering och uppföljning av olika leukemier.  Manuell mikroskopering och utvärdering av leukocyterna är tidskrävande och inte alltid självklar eftersom det krävs personal med hög kompetens och erfarenhet för bedömning av cellerna. En tillförlitlig förklassificering av den manuella differentialräknaren CellaVision DM1200 är därför av stort intresse för att underlätta kvantifiering och klassificering av cellerna. För att se om instrumentets förklassificering påverkades av infärgningen undersöktes färglösningens inverkan genom ändringar i fosfatbuffertens pH. Blodutstryk som härstammade från 10 patientprov färgades in med buffertar med pH 6.0, 6.8 och 7.5. Studien visade på en god positiv korrelation i samtliga pH-justerade buffertar, men med bäst resultat för pH 6.0 och 6.8. CellaVision DM1200:s förklassificering av eosinofila granulocyter påverkades mest av pH ändringar, vilket kan förklaras av deras starkt acidofila granula. 

Abstract [en]

In peripheral blood the composition of leukocytes vary due to diseases and therefore analysis of the cells is critical in diagnosing and monitoring various leukemias. Manual microscopy and evaluation of the leukocytes is time consuming and not always clear, and it requires educated staff with long experience to assess the cells. A reliable automatic pre-classification of the cells with the system CellaVision DM12000 is therefor of great interest to facilitate quantification and classification of leukocytes. To evaluate if the instrument’s pre-classification were affected by staining, changes of the current staining solution used in clinical assessment where made by adjustment of the phosphate buffer pH. Examined blood smears that were descendent from 10 different samples were stained in buffers with pH 6.0, 6.8 and 7.5. This study showed a high positive correlation in all of the pH-adjusted buffers with the best results in pH 6.0 and 6.8. CellaVision DM1200’s pre-classification of eosinophilic granulocytes were most affected by changes in pH, which can be explained by their strongly acidophilic granules. 

Place, publisher, year, edition, pages
2015. , 25 p.
Keyword [sv]
Leukocyter, neutrofila granulocyter, eosinofila granulocyter, basofila granulocyter, monocyter, lymfocyter, omogna celler, automatiserade cellräknare, May Grünwald-Giema, Romanowsky, fosfatbuffert, differentialräkning, CellaVision DM1200
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:hkr:diva-14225OAI: oai:DiVA.org:hkr-14225DiVA: diva2:838537
Educational program
Bachelor of Science in Biomedical Laboratory Science
Supervisors
Examiners
Available from: 2015-08-06 Created: 2015-06-30 Last updated: 2015-08-06Bibliographically approved

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Biomedical Laboratory Science/Technology

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CiteExportLink to record
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