CGGBP1 mitigates cytosine methylation at repetitive DNA sequences
2015 (English)In: BMC Genomics, ISSN 1471-2164, Vol. 16, 390Article in journal (Refereed) Published
Background: CGGBP1 is a repetitive DNA-binding transcription regulator with target sites at CpG-rich sequences such as CGG repeats and Alu-SINEs and L1-LINEs. The role of CGGBP1 as a possible mediator of CpG methylation however remains unknown. At CpG-rich sequences cytosine methylation is a major mechanism of transcriptional repression. Concordantly, gene-rich regions typically carry lower levels of CpG methylation than the repetitive elements. It is well known that at interspersed repeats Alu-SINEs and L1-LINEs high levels of CpG methylation constitute a transcriptional silencing and retrotransposon inactivating mechanism. Results: Here, we have studied genome-wide CpG methylation with or without CGGBP1-depletion. By high throughput sequencing of bisulfite-treated genomic DNA we have identified CGGBP1 to be a negative regulator of CpG methylation at repetitive DNA sequences. In addition, we have studied CpG methylation alterations on Alu and L1 retrotransposons in CGGBP1-depleted cells using a novel bisulfite-treatment and high throughput sequencing approach. Conclusions: The results clearly show that CGGBP1 is a possible bidirectional regulator of CpG methylation at Alus, and acts as a repressor of methylation at L1 retrotransposons.
Place, publisher, year, edition, pages
2015. Vol. 16, 390
IdentifiersURN: urn:nbn:se:uu:diva-256126DOI: 10.1186/s12864-015-1593-2ISI: 000354528700001PubMedID: 25981527OAI: oai:DiVA.org:uu-256126DiVA: diva2:824825