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CGGBP1 mitigates cytosine methylation at repetitive DNA sequences
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Experimental and Clinical Oncology. Uppsala University, Science for Life Laboratory, SciLifeLab.
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2015 (English)In: BMC Genomics, ISSN 1471-2164, Vol. 16, 390Article in journal (Refereed) Published
Abstract [en]

Background: CGGBP1 is a repetitive DNA-binding transcription regulator with target sites at CpG-rich sequences such as CGG repeats and Alu-SINEs and L1-LINEs. The role of CGGBP1 as a possible mediator of CpG methylation however remains unknown. At CpG-rich sequences cytosine methylation is a major mechanism of transcriptional repression. Concordantly, gene-rich regions typically carry lower levels of CpG methylation than the repetitive elements. It is well known that at interspersed repeats Alu-SINEs and L1-LINEs high levels of CpG methylation constitute a transcriptional silencing and retrotransposon inactivating mechanism. Results: Here, we have studied genome-wide CpG methylation with or without CGGBP1-depletion. By high throughput sequencing of bisulfite-treated genomic DNA we have identified CGGBP1 to be a negative regulator of CpG methylation at repetitive DNA sequences. In addition, we have studied CpG methylation alterations on Alu and L1 retrotransposons in CGGBP1-depleted cells using a novel bisulfite-treatment and high throughput sequencing approach. Conclusions: The results clearly show that CGGBP1 is a possible bidirectional regulator of CpG methylation at Alus, and acts as a repressor of methylation at L1 retrotransposons.

Place, publisher, year, edition, pages
2015. Vol. 16, 390
National Category
Medical Genetics
URN: urn:nbn:se:uu:diva-256126DOI: 10.1186/s12864-015-1593-2ISI: 000354528700001PubMedID: 25981527OAI: diva2:824825
Available from: 2015-06-22 Created: 2015-06-22 Last updated: 2015-06-22Bibliographically approved

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Agarwal, PrasoonWiklund, Helena JernbergWestermark, BengtSingh, Umashankar
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Experimental and Clinical OncologyScience for Life Laboratory, SciLifeLabNeuro-Oncology
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