Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Ideonella dechloratans: Investigation of the chlorite dismutase promoter
Karlstad University, Faculty of Health, Science and Technology (starting 2013). University College Leuven Limburg.
2015 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Chlorate and perchlorate pollutions have become a problem in the environment in the last decades. Studies have shown that some bacteria can degrade these substances into unharmful substances such as chloride and molecular oxygen. One of these chlorate degrading bacteria is Ideonella dechloratans that uses chlorate reductase and chlorite dismutase to process chlorate. In the promoter gene sequence of chlorite dismutase there might be regulator sequences such as fumarate and nitrate reductase regulator (FNR) and aerobic respiration control protein (ArcA) that might control the transcription of this enzyme. This promoter sequence was placed in a pBBR1MCS-4-LacZ reporter vector and the possible regulatory sequences were changed through site-directed mutagenesis and tested on activity through beta-galactosidase assays. The changes in the FNR binding sequence gave beta-galactosidase activity that was close to a negative control which might give conclusions that either FNR has an important role or an important part of the promoter was hit. The changes in the ArcA regulator binding sequence did not give such big differences and no certainty can be given if this made important changes to the promoter.

Place, publisher, year, edition, pages
2015. , 36 p.
Keyword [en]
Ideonella dechloratans, chlorite dismutase promoter, site-directed mutagenesis, FNR, ArcA
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kau:diva-36222OAI: oai:DiVA.org:kau-36222DiVA: diva2:817786
Supervisors
Examiners
Available from: 2015-06-25 Created: 2015-06-06 Last updated: 2015-06-25Bibliographically approved

Open Access in DiVA

fulltext(1611 kB)117 downloads
File information
File name FULLTEXT01.pdfFile size 1611 kBChecksum SHA-512
e2dedafe24ebc8e10324dc8570f300de6933096489667377b6372c5e434404a1e06e86b37386ccf7fb77bf10ca72562c1b73200c2e89a7160dc8b8931cb04166
Type fulltextMimetype application/pdf

By organisation
Faculty of Health, Science and Technology (starting 2013)
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 117 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 101 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf