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Bioremediation of hexavalent chromium (VI) by a soil borne bacterium, Enterobacter cloacae B2-DHA
University of Skövde, School of Bioscience. University of Skövde, The Systems Biology Research Centre. Örebro Universtitet. (Physiology, Pharmacology and Toxicology)ORCID iD: 0000-0002-8162-8945
University of Skövde, School of Bioscience. University of Skövde, The Systems Biology Research Centre. (Physiology, Pharmacology and Toxicology)
Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Tathawade, Pune, India.
The Life Science Center, School of Science and Technology, Örebro University, Örebro, Sweden.
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2015 (English)In: Journal of Environmental Science and Health. Part A: Toxic/Hazardous Substances and Environmental Engineering, ISSN 1093-4529, E-ISSN 1532-4117, Vol. 50, no 11, 1136-1147 p.Article in journal (Refereed) Published
Abstract [en]

Chromium and chromium containing compounds are discharged into the nature as waste from anthropogenic activities, such as industries, agriculture, forest farming, mining and metallurgy. Continued disposal of these compounds to the environment leads to development of various lethal diseases in both humans and animals. In this paper, we report a soil borne bacterium, B2-DHA that can be used as a vehicle to effectively remove chromium from the contaminated sources. B2-DHA is resistant to chromium with a MIC value of 1000 µg/mL potassium chromate. The bacterium has been identified as a Gram negative, Enterobacter cloacae based on biochemical characteristics and 16S rRNA gene analysis. TOF-SIMS and ICP-MS analyses confirmed intracellular accumulation of chromium and thus its removal from the contaminated liquid medium. Chromium accumulation in cells was 320 µg/g of cells dry biomass after 120 h exposure and thus it reduced the chromium concentration in the liquid medium by as much as 81%. Environmental scanning electron micrograph revealed the effect of metals on cellular morphology of the isolates. Altogether, our results indicate that B2-DHA has the potential to reduce chromium significantly to safe levels from the contaminated environments and suggest the potential use of this bacterium in reducing human exposure to chromium, hence avoiding poisoning.

Place, publisher, year, edition, pages
Taylor & Francis, 2015. Vol. 50, no 11, 1136-1147 p.
Keyword [en]
Bioremediation, Chromium, Enterobacter cloacae, Human Health, Tannery Effluents, Soil Borne Bacterium
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URN: urn:nbn:se:his:diva-10916DOI: 10.1080/10934529.2015.1047670PubMedID: 26191988ScopusID: 2-s2.0-84937800926OAI: diva2:811261
Available from: 2015-05-11 Created: 2015-05-11 Last updated: 2016-09-07Bibliographically approved
In thesis
1. Bioremediation of Toxic Metals for Protecting Human Health and the Ecosystem
Open this publication in new window or tab >>Bioremediation of Toxic Metals for Protecting Human Health and the Ecosystem
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Heavy metal pollutants, discharged into the ecosystem as waste by anthropogenic activities, contaminate drinking water for millions of people and animals in many regions of the world. Long term exposure to these metals, leads to several lethal diseases like cancer, keratosis, gangrene, diabetes, cardio- vascular disorders, etc. Therefore, removal of these pollutants from soil, water and environment is of great importance for human welfare. One of the possible eco-friendly solutions to this problem is the use of microorganisms that can accumulate the heavy metals from the contaminated sources, hence reducing the pollutant contents to a safe level.

In this thesis an arsenic resistant bacterium Lysinibacillus sphaericus B1-CDA, a chromium resistant bacterium Enterobacter cloacae B2-DHA and a nickel resistant bacterium Lysinibacillus sp. BA2 were isolated and studied. The minimum inhibitory concentration values of these isolates are 500 mM sodium arsenate, 5.5 mM potassium chromate and 9 mM nickel chloride, respectively. The time of flight-secondary ion mass spectrometry and inductively coupled plasma-mass spectroscopy analyses revealed that after 120 h of exposure, the intracellular accumulation of arsenic in B1-CDA and chromium in B2-DHA were 5.0 mg/g dwt and 320 μg/g dwt of cell biomass, respectively. However, the arsenic and chromium contents in the liquid medium were reduced to 50% and 81%, respectively. The adsorption values of BA2 when exposed to nickel for 6 h were 238.04 mg of Ni(II) per gram of dead biomass indicating BA2 can reduce nickel content in the solution to 53.89%. Scanning electron micrograph depicted the effect of these metals on cellular morphology of the isolates. The genetic composition of B1-CDA and B2-DHA were studied in detail by sequencing of whole genomes. All genes of B1-CDA and B2-DHA predicted to be associated with resistance to heavy metals were annotated.

The findings in this study accentuate the significance of these bacteria in removing toxic metals from the contaminated sources. The genetic mechanisms of these isolates in absorbing and thus removing toxic metals could be used as vehicles to cope with metal toxicity of the contaminated effluents discharged to the nature by industries and other human activities.

Place, publisher, year, edition, pages
Örebro: Örebro University, 2016. 80 p.
, Örebro Studies in Life Science, ISSN 1653-3100 ; 15
Heavy Metals, Pollution, Accumulation, Remediation, Human Health, Bacteria, Genome Sequencing, de novo Assembly, Gene Prediction
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Other Biological Topics
urn:nbn:se:his:diva-12885 (URN)978-91-7529-146-8 (ISBN)
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Public defence
2016-09-22, Hus G, sal G 111, Högskolevägen, Skövde, 13:15 (English)
Available from: 2016-09-07 Created: 2016-09-07 Last updated: 2016-09-08Bibliographically approved

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