Change search
ReferencesLink to record
Permanent link

Direct link
Insights on the Evolution of Mycoparasitism from the Genome of Clonostachys rosea
Show others and affiliations
2015 (English)In: Genome Biology and Evolution, ISSN 1759-6653, Vol. 7, no 2, 465-480 p.Article in journal (Refereed) Published
Abstract [en]

Clonostachys rosea is a mycoparasitic fungus that can control several important plant diseases. Here, we report on the genome sequencing of C. rosea and a comparative genome analysis, in order to resolve the phylogenetic placement of C. rosea and to study the evolution of mycoparasitism as a fungal lifestyle. The genome of C. rosea is estimated to 58.3 Mb, and contains 14,268 predicted genes. A phylogenomic analysis shows that C. Tosco clusters as sister taxon to plant pathogenic Fusarium species, with mycoparasitic/saprotrophic Tfichoderma species in an ancestral position. A comparative analysis of gene family evolution reveals several distinct differences between the included mycoparasites. Clonostachys rosea contains significantly more ATP-binding cassette (ABC) transporters, polyketide synthases, cytochrome P450 monooxygenases, pectin lyases, glucose-methanol-choline oxidoreductases, and lytic polysaccharide monooxygenases compared with other fungi in the Hypocreales. Interestingly, the increase of ABC transporter gene number in C. rosea is associated with phylogenetic subgroups B (multidrug resistance proteins) and G (pleiotropic drug resistance transporters), whereas an increase in subgroup C (multidrug resistance-associated proteins) is evident in Tfichoderma virens. In contrast with mycoparasitic Tfichoderma species, C. rosea contains very few chitinases. Expression of six group B and group G ABC transporter genes was induced in C. rosea during exposure to the Fusafium mycotoxin zearalenone, the fungicide Boscalid or metabolites from the biocontrol bacterium Pseudomonas chiororaphis. The data suggest that tolerance toward secondary metabolites is a prominent feature in the biology of C. rosea.

Place, publisher, year, edition, pages
2015. Vol. 7, no 2, 465-480 p.
Keyword [en]
ABC transporter, biological control, Bionectria ochroleuca, fungicide, phylogeny, polyketide synthase
National Category
Evolutionary Biology Genetics
URN: urn:nbn:se:uu:diva-251844DOI: 10.1093/gbe/evu292ISI: 000351607800005PubMedID: 25575496OAI: diva2:807835
Available from: 2015-04-24 Created: 2015-04-24 Last updated: 2015-04-24Bibliographically approved

Open Access in DiVA

fulltext(1430 kB)85 downloads
File information
File name FULLTEXT01.pdfFile size 1430 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Nygren, Kristiina
By organisation
Department of Medical Biochemistry and Microbiology
In the same journal
Genome Biology and Evolution
Evolutionary BiologyGenetics

Search outside of DiVA

GoogleGoogle Scholar
Total: 85 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 332 hits
ReferencesLink to record
Permanent link

Direct link