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The investigation of the biotransformation products formed by Cunninghamella elegans for different classes of drugs by the use of UPLC Q-TOF MS
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Analytical Pharmaceutical Chemistry.
2015 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

The fungus Cunninghamella elegans has in many studies shown to have abiotransformation similar to the metabolism of mammals. If the biotransformation isgeneral, it enables the production of metabolites by the fungus and the use asreference material. The purpose of the project were to examine whether themetabolic process of C. elegans is general, with respect to the formation ofglucosides, and can be applied to different classes of drugs. During the project, theanalyses were performed on a UPLC Q-TOF, run in both MSE and MSMS mode. Themobile phase used consisted of MeOH and 0.1 % formic acid in MQ water. Toincrease the concentration of possible glucosides, the samples were subjected to anacidic or alkaline SPE. Glucosides were detected in the fungal incubates of diclofenac,buprenorphine, norbuprenorphine and oxazepam. For diclofenac, besides twodifferent glucosides (diclofenac glucoside and hydroxylated diclofenac glucoside), ahydroxylated metabolite and a hydroxylated metabolite conjugated with sulfate werediscovered. In the samples containing buprenorphine, the phase I metabolitenorbuprenorphine was also encountered. Further, in the fungal incubates ofdexamethasone a defluorinated metabolite was identified, which is a metabolicpathway never before described for C. elegans.ISSN: 1650

Place, publisher, year, edition, pages
2015. , 28 p.
Series
UPTEC K, ISSN 1650-8297 ; 14030
Keyword [en]
UPLC Q-TOF MS, Glucosides, Glucuronides, TEMPO reaction, Cunninghamella elegans
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-248510OAI: oai:DiVA.org:uu-248510DiVA: diva2:802328
External cooperation
Statens Veterinärmedicinska Anstalt
Educational program
Master Programme in Chemical Engineering
Supervisors
Examiners
Available from: 2015-06-23 Created: 2015-03-30 Last updated: 2015-06-23Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
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