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The adhesive protein invasin of Yersinia pseudotuberculosis induces neutrophil extracellular traps via β1 integrins
Umeå University, Faculty of Medicine, Department of Clinical Microbiology. Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Clinical Bacteriology. Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
2015 (English)In: Microbes and infection, ISSN 1286-4579, E-ISSN 1769-714X, Vol. 17, no 5, 327-336 p.Article in journal (Refereed) Published
Abstract [en]

Yersinia pseudotuberculosis adhesive protein invasin is crucial for the bacteria to cross the intestine epithelium by binding to β1 integrins on M-cells and gaining access to the underlying tissues. After the crossing invasin can bind to β1 integrins on other cell surfaces, however effector proteins delivered by the type III secretion system Y. pseudotuberculosis efficiently inhibit potential immune responses induced by this interaction. Here, we use mutant Y. pseudotuberculosis strains lacking the type III secretion system and additionally invasin-expressing Escherichia coli to analyze neutrophil responses towards invasin. Our data reveals that invasin induces production of reactive oxygen species and release of chromatin into the extracellular milieu, which we confirmed to be neutrophil extracellular traps by immunofluorescence microscopy. This was mediated through β1 integrins and was dependent on both the production of reactive oxygen species and signaling through phosphoinositide 3-kinase. We therefore have gained insight into a potential role of integrins in inflammation and infection clearance that has not previously been described, suggesting that targeting of β1 integrins could be utilized as an adjunctive therapy against yersiniosis.

Place, publisher, year, edition, pages
2015. Vol. 17, no 5, 327-336 p.
Keyword [en]
Neutrophil, Yersinia, Invasin, Integrin, NET
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-101195DOI: 10.1016/j.micinf.2014.12.014ISI: 000355037600002PubMedID: 25576025OAI: oai:DiVA.org:umu-101195DiVA: diva2:797697
Available from: 2015-03-24 Created: 2015-03-24 Last updated: 2017-12-04Bibliographically approved

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