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Differential vital staining of normal fibroblasts and melanoma cells by an anionic conjugated polyelectrolyte
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, The Institute of Technology.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Health Sciences. Institute of Automatic Control, Silesian University of of TechnologyGliwice, Poland.
Linköping University, Department of Physics, Chemistry and Biology, Biomolecular and Organic Electronics. Linköping University, The Institute of Technology. Carl Zeiss AB, Sweden.
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2015 (English)In: Cytometry Part A, ISSN 1552-4922, E-ISSN 1552-4930, Vol. 87, no 3, 262-272 p.Article in journal (Refereed) Published
Abstract [en]

Molecular probes for imaging of live cells are of great interest for studying biological and pathological processes. The anionic luminescent conjugated polythiophene (LCP) polythiophene acetic acid (PTAA), has previously been used for vital staining of cultured fibroblasts as well as transformed cells with results indicating differential staining due to cell phenotype. Herein, we investigated the behavior of PTAA in two normal and five transformed cells lines. PTAA fluorescence in normal cells appeared in a peripheral punctated pattern whereas the probe was more concentrated in a one-sided perinuclear localization in the five transformed cell lines. In fibroblasts, PTAA fluorescence was initially associated with fibronectin and after 24 h partially localized to lysosomes. The uptake and intracellular target in malignant melanoma cells was more ambiguous and the intracellular target of PTAA in melanoma cells is still elusive. PTAA was well tolerated by both fibroblasts and melanoma cells, and microscopic analysis as well as viability assays showed no signs of negative influence on growth. Stained cells maintained their proliferation rate for at least 12 generations. Although the probe itself was nontoxic, photoinduced cellular toxicity was observed in both cell lines upon irradiation directly after staining. However, no cytotoxicity was detected when the cells were irradiated 24 h after staining, indicating that the photoinduced toxicity is dependent on the cellular location of the probe. Overall, these studies certified PTAA as a useful agent for vital staining of cells, and that PTAA can potentially be used to study cancer-related biological and pathological processes.

Place, publisher, year, edition, pages
Wiley: 12 months , 2015. Vol. 87, no 3, 262-272 p.
Keyword [en]
Conjugated polyelectrolyte; Fibroblast; Fluorescence; Luminescent conjugated polythiophene; Melanoma; Photoinduced toxicity
National Category
Structural Biology Cell and Molecular Biology
URN: urn:nbn:se:liu:diva-115887DOI: 10.1002/cyto.a.22627ISI: 000349984200009PubMedID: 25605326ScopusID: 2-s2.0-84923259526OAI: diva2:797146
Available from: 2015-03-23 Created: 2015-03-23 Last updated: 2016-02-15
In thesis
1. Poly-and oligothiophenes: Optical probes for multimodal fluorescent assessment of biological processes
Open this publication in new window or tab >>Poly-and oligothiophenes: Optical probes for multimodal fluorescent assessment of biological processes
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

One interesting class of molecules in the research field of imaging biological processes is luminescent conjugated polythiophenes, LCPs. These fluorescent probes have a flexible backbone consisting of repetitive thiophene units. Due to this backbone, the probes possess unique abilities to give rise to different spectral signatures depending on their target and environment. LCPs are a polydispersed material meaning there is an uneven distribution of lengths of the probe. Recently, monodispersed chemically well-defined material denoted luminescent conjugated oligothiophenes, LCOs, with an exact number of repetitive units and distinct sidechain functionalities along the backbone has been developed. LCOs have the advantages of being smaller which leads to higher ability to cross the blood brain barrier. The synthesis of minor chemical alterations is also more simplified due to the well-defined materials.

During my doctoral studies I have used both LCPs and LCOs to study biological processes such as conformational variation of protein aggregates in prion diseases and cellular uptake in normal cells and cancer cells. The research has generally been based on the probes capability to emit light upon irradiation and the interaction with their targets has mainly been assessed through variations in fluorescence intensity, emission-and excitation profiles and fluorescence lifetime decay. These studies verified the utility of LCPs and LCOs for staining and discrimination of both prion strains and cell phenotypes. The results also demonstrated the pronounced influence minor chemical modifications have on the LCO´s staining capacity.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2015. 54 p.
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1693
National Category
Chemical Sciences Cell and Molecular Biology
urn:nbn:se:liu:diva-121815 (URN)10.3384/diss.diva-121815 (DOI)978-91-7685-986-5 (print) (ISBN)
Public defence
2015-11-06, Planck, Fysikhuset, Campus Valla, Linköping, 10:15 (Swedish)
Available from: 2015-10-07 Created: 2015-10-07 Last updated: 2015-10-07Bibliographically approved

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Magnusson, KarinAppelqvist, HannaCieslar-Pobuda, ArturWigenius, JensKarlsson, ThommieLos, Marek JanKågedal, BertilJonasson, Jon
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