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Detection and Isolation of Sindbis Virus from Mosquitoes Captured During an Outbreak in Sweden, 2013
Umeå University, Faculty of Medicine, Department of Clinical Microbiology.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology.
Umeå University, Faculty of Medicine, Department of Medical Biosciences.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology.
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2015 (English)In: Vector Borne and Zoonotic Diseases, ISSN 1530-3667, E-ISSN 1557-7759, Vol. 15, no 2, 133-140 p.Article in journal (Refereed) Published
Abstract [en]

Mosquito-borne alphaviruses have the potential to cause large outbreaks throughout the world. Here we investigated the causative agent of an unexpected Sindbis virus (SINV) outbreak during August-September, 2013, in a previously nonendemic region of Sweden. Mosquitoes were collected using carbon dioxide-baited CDC traps at locations close to human cases. The mosquitoes were initially screened as large pools by SINV-specific quantitative RT-PCR, and the SINV-positive mosquitoes were species determined by single-nucleotide polymorphism (SNP) analysis, followed by sequencing the barcoding region of the cytochrome oxidase I gene. The proportion of the collected mosquitoes was determined by a metabarcoding strategy. By using novel strategies for PCR screening and genetic typing, a new SINV strain, Lovanger, was isolated from a pool of 1600 mosquitoes composed of Culex, Culiseta, and Aedes mosquitoes as determined by metabarcoding. The SINV-positive mosquito Culiseta morsitans was identified by SNP analysis and sequencing. After whole-genome sequencing and phylogenetic analysis, the SINV Lovanger isolate was shown to be most closely similar to recent Finnish SINV isolates. In conclusion, within a few weeks, we were able to detect and isolate a novel SINV strain and identify the mosquito vector during a sudden SINV outbreak.

Place, publisher, year, edition, pages
2015. Vol. 15, no 2, 133-140 p.
Keyword [en]
Barcoding, Mosquito, Isolation, Virus, Sindbis
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:umu:diva-100963DOI: 10.1089/vbz.2014.1717ISI: 000349682500007PubMedID: 25700044OAI: oai:DiVA.org:umu-100963DiVA: diva2:796170
Available from: 2015-03-18 Created: 2015-03-16 Last updated: 2017-12-04Bibliographically approved

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Bergqvist, JoakimForsman, OscarLarsson, PärNäslund, JonasEngdahl, CeciliaGylfe, ÅsaAhlm, ClasEvander, MagnusBucht, Göran
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Department of Clinical MicrobiologyDepartment of Medical BiosciencesDepartment of ChemistryArctic Research Centre at Umeå University
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Vector Borne and Zoonotic Diseases
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