Change search
ReferencesLink to record
Permanent link

Direct link
Affinity proteomics discovers decreased levels of AMFR in plasma from Osteoporosis patients
KTH Royal Inst Technol, Affin Prote, SciLifeLab, Solna, Sweden.
KTH Royal Inst Technol, Affin Prote, SciLifeLab, Solna, Sweden.
KTH Royal Inst Technol, Affin Prote, SciLifeLab, Solna, Sweden.
KTH Royal Inst Technol, Affin Prote, SciLifeLab, Solna, Sweden.
Show others and affiliations
2016 (English)In: PROTEOMICS - Clinical Applications, ISSN 1862-8346, E-ISSN 1862-8354, Vol. 10, no 6, 681-690 p.Article in journal (Refereed) Published
Abstract [en]

PURPOSE:: Affinity proteomic approaches by antibody bead arrays enable multiplexed analysis of proteins in body fluids. In the presented study, we investigated blood plasma within osteoporosis to discovery differential protein profiles and to propose novel biomarkers candidates for subsequent studies.

EXPERIMENTAL DESIGN:: Starting with 4608 antibodies and plasma samples from 22 women for an untargeted screening, a set of 72 proteins were suggested for further analysis. Complementing these with targets from literature and other studies, a targeted bead array of was built to profile for 92 proteins in plasma samples of 180 women from two independent population-based studies.

RESULTS:: Differential profiles between osteoporosis patients and matched controls were discovered for 12 proteins in at least one of the two study sets. Among these targets, the levels of Autocrine Motility Factor Receptor (AMFR) were concordantly lower in plasma of female osteoporosis patients. Subsequently, verification of anti-AMFR antibody selectivity was conducted using high-density peptide and protein arrays, and Western blotting.

CONCLUSIONS AND CLINICAL RELEVANCE:: Further validation in additional study sets will be needed to determine the clinical value of the observed decrease in AMFR plasma levels in osteoporosis patients, but AMFR may aid our understanding of disease mechanisms and could support existing tools for diagnosis and monitoring of patient mobility within osteoporosis.

Place, publisher, year, edition, pages
2016. Vol. 10, no 6, 681-690 p.
Keyword [en]
Antibody bead arrays; Biomarker discovery; Osteoporosis; Plasma
National Category
Medicinal Chemistry
URN: urn:nbn:se:uu:diva-245059DOI: 10.1002/prca.201400167ISI: 000383846700007PubMedID: 25689831OAI: diva2:790339
Science for Life Laboratory - a national resource center for high-throughput molecular bioscienceKnut and Alice Wallenberg Foundation
Available from: 2015-02-24 Created: 2015-02-24 Last updated: 2016-11-24Bibliographically approved

Open Access in DiVA

fulltext(1616 kB)15 downloads
File information
File name FULLTEXT01.pdfFile size 1616 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Michaëlsson, Karl
By organisation
OrthopaedicsUCR-Uppsala Clinical Research Center
In the same journal
PROTEOMICS - Clinical Applications
Medicinal Chemistry

Search outside of DiVA

GoogleGoogle Scholar
Total: 15 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 183 hits
ReferencesLink to record
Permanent link

Direct link