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STED microscopy: towards broadened use and scope of applications
KTH, School of Engineering Sciences (SCI), Applied Physics, Cell Physics. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0002-5584-9170
KTH, School of Engineering Sciences (SCI), Applied Physics, Experimental Biomolecular Physics.
2014 (English)In: Current opinion in chemical biology, ISSN 1367-5931, E-ISSN 1879-0402, Vol. 20, no 1, 127-133 p.Article, review/survey (Refereed) Published
Abstract [en]

High resolution Stimulated Emission Depletion (STED) microscopy has been demonstrated for fundamental studies in cells, living tissue and organisms. Today, a major trend in the STED technique development is to make the instruments simpler and more user-friendly, without compromising performance. This has become possible by new low-cost, turn-key laser technology and by implementing specifically designed phase plates and polarization elements, extending and simplifying the shaping of the laser beam profiles. These simpler and cheaper realizations of STED are now becoming more broadly available. In parallel with the continuous development of sample preparation and fluorophore reporter molecules ultimately setting the limit of the image quality, contrast and resolution, we can thus expect a significant increase in the use of STED, in science as well as for clinical and drug development purposes.

Place, publisher, year, edition, pages
Elsevier, 2014. Vol. 20, no 1, 127-133 p.
Keyword [en]
Field Optical Nanoscopy, Stimulated-Emission, Fluorescence Microscopy, Superresolution Microscopy, Dendritic Spines, Brain-Slices, Resolution, Cell, Proteins, Light
National Category
Biochemistry and Molecular Biology Biophysics
Research subject
Biological Physics
URN: urn:nbn:se:kth:diva-159636DOI: 10.1016/j.cbpa.2014.06.004ISI: 000347500100018PubMedID: 24983537ScopusID: 2-s2.0-84903542533OAI: diva2:786483
Swedish Cancer Society, CAN-2011/654Knut and Alice Wallenberg Foundation, KAW 2011.0218Swedish Research Council, VR 2013-6041Science for Life Laboratory - a national resource center for high-throughput molecular bioscience

QC 20150205

Available from: 2015-02-05 Created: 2015-02-05 Last updated: 2016-03-10Bibliographically approved

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