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BDNF Increases Survival and Neuronal Differentiation of Human Neural Precursor Cells Cotransplanted with a Nanofiber Gel to the Auditory Nerve in a Rat Model of Neuronal Damage
Karolinska University Hospital, Sweden; Karolinska University Hospital, Sweden; Capital Medical University, Peoples R China.
Karolinska University Hospital, Sweden; Karolinska University Hospital, Sweden.
Karolinska University Hospital, Sweden; Karolinska University Hospital, Sweden.
Lund University, Sweden.
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2014 (English)In: BioMed Research International, ISSN 2314-6133, E-ISSN 2314-6141, Vol. 2014, 1-11 p.Article in journal (Refereed) Published
Abstract [en]

Objectives. To study possible nerve regeneration of a damaged auditory nerve by the use of stem cell transplantation. Methods. We transplanted HNPCs to the rat AN trunk by the internal auditory meatus (IAM). Furthermore, we studied if addition of BDNF affects survival and phenotypic differentiation of the grafted HNPCs. A bioactive nanofiber gel (PA gel), in selected groups mixed with BDNF, was applied close to the implanted cells. Before transplantation, all rats had been deafened by a round window niche application of beta-bungarotoxin. This neurotoxin causes a selective toxic destruction of the AN while keeping the hair cells intact. Results. Overall, HNPCs survived well for up to six weeks in all groups. However, transplants receiving the BDNF-containing PA gel demonstrated significantly higher numbers of HNPCs and neuronal differentiation. At six weeks, a majority of the HNPCs had migrated into the brain stem and differentiated. Differentiated human cells as well as neurites were observed in the vicinity of the cochlear nucleus. Conclusion. Our results indicate that human neural precursor cells (HNPC) integration with host tissue benefits from additional brain derived neurotrophic factor (BDNF) treatment and that these cells appear to be good candidates for further regenerative studies on the auditory nerve (AN).

Place, publisher, year, edition, pages
Hindawi Publishing Corporation , 2014. Vol. 2014, 1-11 p.
National Category
Clinical Medicine
URN: urn:nbn:se:liu:diva-114033DOI: 10.1155/2014/356415ISI: 000347810500001PubMedID: 25243135OAI: diva2:786447
Available from: 2015-02-05 Created: 2015-02-05 Last updated: 2015-06-10

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Olivius, Petri
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Division of NeuroscienceFaculty of Health SciencesDepartment of Otorhinolaryngology in Linköping
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