Change search
ReferencesLink to record
Permanent link

Direct link
An in vitro Study of Drug-induced Degranulation of Human HMC-1 Mast Cells and Rat RBL-2H3 Cells
Umeå University, Faculty of Medicine, Department of Odontology, School of Dentistry.
Umeå University, Faculty of Medicine, Department of Odontology, School of Dentistry.
2014 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Mast cells and basophils participate in biological responses such as inflammation and allergic reactions and contain potent mediators such as histamine and cytokines. Some drugs, for example opioids, are known to trigger mast cell degranulation with the release of immunogenic mediators that may cause severe side effects.

The aim of this study was to investigate if the human mast cell line HMC-1 is a useful model to study codeine-induced degranulation in vitro. The rat basophilic leukemia RBL-2H3 cell line was used as a reference. Time- and concentration-dependent effects of codeine, compound 48/80, and A23187 on the degranulation of HMC-1 cells and RBL-2H3 cells were investigated by measuring extracellular levels of β-hexosaminidase and [3H]serotonin.

Neither codeine nor compound 48/80 produced any significant basophil or mast cell degranulation, whereas the calcium ionophore A23187 triggered a degranulation of both cell lines.

In conclusion, none of the cell lines are useful as relevant, robust and reproducible in vitro models to study drug-induced mast cell degranulation.

Place, publisher, year, edition, pages
National Category
Medical and Health Sciences
URN: urn:nbn:se:umu:diva-97850OAI: diva2:777071
Educational program
Dentistry Programme
Available from: 2015-03-11 Created: 2015-01-08 Last updated: 2015-03-11Bibliographically approved

Open Access in DiVA

fulltext(466 kB)304 downloads
File information
File name FULLTEXT01.pdfFile size 466 kBChecksum SHA-512
Type fulltextMimetype application/pdf

By organisation
School of Dentistry
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 304 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 104 hits
ReferencesLink to record
Permanent link

Direct link