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Contribution of Priority PAHs and POPs to Ah Receptor-Mediated Activities in Sediment Samples from the RiverElbe Estuary, Germany
KIT – Karlsruhe Institute of Technology, Institute of Toxicology and Genetics, Karlsruhe, Germany.
RWTH Aachen University, Institute for Environmental Research, Aachen, Germany. (MTM)ORCID iD: 0000-0002-2356-6686
University of Heidelberg, Centre of Organismal Studies, Heidelberg, Germany.
Toxicology Centre, University of Saskatchewan, Saskatoon, Canada.
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2013 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 8, no 10, e7596Article in journal (Refereed) Published
Abstract [en]

The estuary of the River Elbe between Hamburg and the North Sea (Germany) is a sink for contaminated sediment and suspended particulate matter (SPM). One major concern is the effect of human activities on the hydrodynamics, particularlythe intensive dredging activities in this area that may result in remobilization of sediment-bound pollutants. The aim of this study was to identify pollutants contributing to the toxicological risk associated with re-suspension of sediments in the Elbe Estuary by use of an effect-directed analysis that combines chemical and biological analyses in with specific fractionation techniques. Sediments were collected from sites along the Elbe Estuary and a site from a small harbor basin of the Elbe Estuary that is known to be polluted. The sixteen priority EPA-PAHs were quantified in organic extracts of sediments. In addition, dioxin equivalents of sediments were investigated by use of the 7-ethoxyresorufin O-deethylase assay with RTL-W1 cells and the Ah receptor-mediated luciferase transactivation assay with H4IIE-luc cells. Quantification of the 16 priorityPAHs revealed that sediments were moderately contaminated at all of the sites in the Elbe River Estuary (,0.02–0.906 mg/gdw). Sediments contained relatively small concentrations of dioxin equivalents (Bio-TEQ) with concentrations ranging from15.5 to 322 pg/g dw, which were significantly correlated with dioxin equivalents calculated based on toxicity referencevalues and concentrations of PAH. The concentration of Bio-TEQ at the reference site exceeded 200,000 pg/g dw. In apotency balance the 16 PAHs explained between 47 and 118% of the Bio-TEQ in the luciferase assay, which can be explained by the constant input of PAHs bound to SPM from the upper course of the Elbe River into its estuary. Successful identification of a significant portion of dioxin-like activity to priority PAHs in complex environmental samples such assediments has rarely been reported.

Place, publisher, year, edition, pages
San Francisco, USA: Public Library Service , 2013. Vol. 8, no 10, e7596
National Category
Environmental Sciences
Research subject
Enviromental Science
URN: urn:nbn:se:oru:diva-40148DOI: 10.1371/journal.pone.0075596ISI: 000325819400013PubMedID: 24146763ScopusID: 2-s2.0-84885403922OAI: diva2:776141
Available from: 2015-01-07 Created: 2015-01-07 Last updated: 2015-10-19Bibliographically approved

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