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Detection of Rotavirus Using Padlock Probes and Rolling Circle Amplification
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology and Infectious Medicine.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology and Infectious Medicine.
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2014 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 9, no 11, e111874- p.Article in journal (Refereed) Published
Abstract [en]

Rotavirus infections are one of the most common reasons for hospitalizations due to gastrointestinal diseases. Rotavirus is often diagnosed by latex agglutination assay, chromatography immunoassay, or by electron microscopy, which are all quite insensitive. Reverse transcription polymerase chain reaction, on the other hand, is very sensitive to variations at the genomic level. We developed a novel assay based on a set of 58 different padlock probes with a detection limit of 1,000 copies. Twenty-two patient samples were analyzed and the assay showed high concordance with a PCR-based assay. In summary, we present a new assay for sensitive and variation tolerant detection of rotavirus.

Place, publisher, year, edition, pages
2014. Vol. 9, no 11, e111874- p.
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Infectious Medicine
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URN: urn:nbn:se:uu:diva-239368DOI: 10.1371/journal.pone.0111874ISI: 000344402000115OAI: oai:DiVA.org:uu-239368DiVA: diva2:774817
Available from: 2014-12-29 Created: 2014-12-22 Last updated: 2017-12-05Bibliographically approved

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