A method to map changes in bacterial surface composition induced by regulatory RNAs in Escherichia coli and Staphylococcus aureus
2014 (English)In: Biochimie, ISSN 0300-9084, E-ISSN 1638-6183, Vol. 106C, 175-179 p.Article in journal (Refereed) Published
We have adapted a method to map cell surface proteins and to monitor the effect of specific regulatory RNAs on the surface composition of the bacteria. This method involves direct labeling of surface proteins of living bacteria using fluorescent dyes and a subsequent separation of the crude extract by 2D gel electrophoresis. The strategy yields a substantial enrichment in surface proteins over cytoplasmic proteins. We validated this method by monitoring the effect of the regulatory RNA MicA in Escherichia colt, which regulates the synthesis of several outer membrane proteins, and highlighted the role of Staphylococcus aureus RNAIII for the maintenance of cell wall integrity.
Place, publisher, year, edition, pages
2014. Vol. 106C, 175-179 p.
Non-coding RNAs, DIGE, Surface proteins, Post-transcriptional regulation
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-238409DOI: 10.1016/j.biochi.2014.07.011ISI: 000344434700021PubMedID: 25046628OAI: oai:DiVA.org:uu-238409DiVA: diva2:773111