An azide functionalized oligothiophene ligand - A versatile tool for multimodal detection of disease associated protein aggregates
2015 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 63, 204-211 p.Article in journal (Refereed) Published
Ligands for identifying protein aggregates are of great interest as such deposits are the pathological hallmark of a wide range of severe diseases including Alzheimers and Parkinsons disease. Here we report the synthesis of an azide functionalized fluorescent pentameric oligothiophene that can be utilized as a ligand for multimodal detection of disease-associated protein aggregates. The azide functionalization allows for attachment of the ligand to a surface by conventional click chemistry without disturbing selective interaction with protein aggregates and the oligothiophene-aggregate interaction can be detected by fluorescence or surface plasmon resonance. In addition, a methodology where the oligothiophene ligand is employed as a capturing molecule selective for aggregated proteins in combination with an antibody detecting a distinct peptide/protein is also presented. We foresee that this methodology will offer the possibility to create a variety of multiplex sensing systems for sensitive and selective detection of protein aggregates, the pathological hallmarks of several neurodegenerative diseases.
Place, publisher, year, edition, pages
Elsevier , 2015. Vol. 63, 204-211 p.
Protein aggregates; Oligothiophene; Fluorescence; Surface plasmon resonance; Click chemistry
Chemical Sciences Biological Sciences
IdentifiersURN: urn:nbn:se:liu:diva-112169DOI: 10.1016/j.bios.2014.07.042ISI: 000343337000030PubMedID: 25089818OAI: oai:DiVA.org:liu-112169DiVA: diva2:764291
Funding Agencies|Swedish Foundation for Strategic Research; Ehrling Persson Foundation; ERC Starting Independent Researcher grant (Project: MUMID)2014-11-182014-11-182015-10-27