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A Phage Display Screening Derived Peptide with Affinity for the Adeninyl Moiety
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences. (Linnaeus University Centre for Biomaterials Chemistry)
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science. (BMC)
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences. (BMC ; BBCL)
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences. Uppsala University. (BMC ; BBCL)ORCID iD: 0000-0002-0407-6542
2014 (English)In: Biosensors, ISSN 2079-6374, Vol. 4, no 2, p. 137-149Article in journal (Refereed) Published
Abstract [en]

Phage display screening of a surface-immobilized adenine derivative led to the identification of a heptameric peptide with selectivity for adenine as demonstrated through quartz crystal microbalance (QCM) studies. The peptide demonstrated a concentration dependent affinity for an adeninyl moiety decorated surface (KD of 968 ± 53.3 μM), which highlights the power of piezoelectric sensing in the study of weak interactions. 

Place, publisher, year, edition, pages
2014. Vol. 4, no 2, p. 137-149
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
Identifiers
URN: urn:nbn:se:lnu:diva-37248DOI: 10.3390/bios4020137Scopus ID: 2-s2.0-84902317201OAI: oai:DiVA.org:lnu-37248DiVA, id: diva2:749541
Available from: 2014-09-24 Created: 2014-09-24 Last updated: 2017-12-05Bibliographically approved
In thesis
1. QCM-based sensing using biological and biomimetic interfaces
Open this publication in new window or tab >>QCM-based sensing using biological and biomimetic interfaces
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The objective of this thesis was to explore novel approaches for studying molecular recognition at biological and biomimetic surfaces using the quartz crystal microbalance (QCM) biosensor technique. The first two papers focused on the synthesis and study of biotin selective polymer films prepared using the molecularly imprinted polymer (MIP) technique. Control over polymer structure is of importance for sensor reproducibility and sensitivity, and was addressed in Paper I where a simple strategy for fabricating uniform thin biotin imprinted polymer films was employed. In Paper II the binding of biotin moieties to thin (3-5 nm) biomimetic polymer films was examined and consequences for sensor performance discussed. The potential for using QCM as a tool for assessing the binding of small peptides derived from phage display screening was presented Paper III. Here, screening of a phage peptide library against immobilized adenine resulted in candidate peptides that were studied using this technique. In Paper IV a whole cell-based biosensor was developed for studying interactions with cell membrane-incorporated targets. Epithelial cancer cells, SKOV3, were attached to QCM sensor chips and the binding of the monoclonal antibody HerceptinTM was studied. This approach demonstrates the potential of using QCM to study binding to membrane-incorporated targets, an alternative to assays based upon immobilized receptor structures lacking their natural context.

Place, publisher, year, edition, pages
Växjö: Linnaeus University Press, 2014
Series
Linnaeus University Dissertations ; 192/2014
Keyword
Quartz crystal microbalance, molecularly imprinted polymers, phage display, interactions, artificial receptors, recognition, selectivity
National Category
Chemical Sciences
Identifiers
urn:nbn:se:lnu:diva-37514 (URN)978-91-87925-20-7 (ISBN)
Public defence
2014-10-31, N2007, Smålandsgatan 26B, Kalmar, 09:30 (English)
Opponent
Supervisors
Available from: 2014-10-08 Created: 2014-10-07 Last updated: 2014-10-08Bibliographically approved

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