3D image deconvolution in light microscopy: theory and practice
2000 (English)Report (Other academic)
The objective of this study is to investigate the use of deconvolution for improving the three-dimensional resolution in both confocal and wide-field light microscopy. We start by assessing the theoretical limits of the deconvolution by defining and calculating a characteristic function which describes the imaging-deconvolution process. We continue by suggesting a variant of inverse filtering for deconvolving real noisy data and describe the application of the method to a confocal recording of a biological preparation. The result is comparable with that obtainable by Wiener filtering but we can avoid the problem of finding a best value of the parameter. We also describe a way of determining the optical transfer function of the microscope.
Place, publisher, year, edition, pages
KTH Royal Institute of Technology, 2000. , 18 p.
Light microscopy, 3D imaging, deconvolution, characteristic function
IdentifiersURN: urn:nbn:se:kth:diva-150954OAI: oai:DiVA.org:kth-150954DiVA: diva2:747276
QC 201409182014-09-162014-09-112014-09-18Bibliographically approved