A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications
2014 (English)In: Chemical Communications, ISSN 1359-7345, E-ISSN 1364-548X, Vol. 50, no 65, 9134-9137 p.Article in journal (Refereed) Published
A general combined purification and immobilization method to facilitate biocatalytic process development is presented. The support material, EziG (TM), is based on controlled porosity glass (CPG) or polymer-coated versions thereof (HybCPG) and binds protein affinity tags. Biocatalytic reactions in aqueous and organic media with seven enzymes of biocatalytic interest are shown.
Place, publisher, year, edition, pages
Royal Society of Chemistry, 2014. Vol. 50, no 65, 9134-9137 p.
Chemical Sciences Organic Chemistry
Research subject Organic Chemistry
IdentifiersURN: urn:nbn:se:su:diva-107099DOI: 10.1039/c4cc02605eISI: 000339930400026OAI: oai:DiVA.org:su-107099DiVA: diva2:743938
FunderSwedish Research CouncilKnut and Alice Wallenberg Foundation