Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Studying the Glycan Moiety of RNase B by Means of Raman and Raman Optical Activity
Stockholm University, Faculty of Science, Department of Organic Chemistry.
Stockholm University, Faculty of Science, Department of Organic Chemistry.
2014 (English)In: ChemPhysChem, ISSN 1439-4235, E-ISSN 1439-7641, Vol. 15, no 11, 2252-2254 p.Article in journal (Refereed) Published
Abstract [en]

Raman and Raman optical activity (ROA) spectroscopy are used to study the solution-phase structure of the glycan moiety of the protein ribonuclease B (RNase B). Spectral data of the intact glycan moiety of RNase B is obtained by subtracting high-quality spectral data of RNase A, the non-glycosylated form of the RNase, from the spectra of the glycoprotein. The remaining difference spectra are compared to spectra generated from Raman and ROA data of the constituent disaccharides of the RNase glycan, achieving convincing spectral overlap. The results show that ROA spectroscopy is able to extract detailed spectral data of the glycan moieties of proteins, provided that the non-glycosylated isoform is available. Furthermore, good comparison between the full glycan spectrum and the regenerated spectra based on the disaccharide data lends great promise to ROA as a tool for the solution-phase structural analysis of this structurally elusive class of biomolecules.

Place, publisher, year, edition, pages
2014. Vol. 15, no 11, 2252-2254 p.
Keyword [en]
carbohydrates, glycoproteins, Raman optical activity, Raman spectroscopy, ribonuclease
National Category
Chemical Sciences Physical Sciences
Identifiers
URN: urn:nbn:se:su:diva-107111DOI: 10.1002/cphc.201402029ISI: 000340175800009OAI: oai:DiVA.org:su-107111DiVA: diva2:743339
Funder
Swedish Research Council
Note

AuthorCount:4;

Available from: 2014-09-03 Created: 2014-09-03 Last updated: 2017-12-05Bibliographically approved

Open Access in DiVA

fulltext(234 kB)20 downloads
File information
File name FULLTEXT01.pdfFile size 234 kBChecksum SHA-512
fbce41df93415706c1ec4840f67a3e00dede96c8fd34b2e80235f3d720ebe166e085bd67bc33f89cc944b6eb03f35a350808554b61aaa82b5c4562503796a95c
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Widmalm, Göran
By organisation
Department of Organic Chemistry
In the same journal
ChemPhysChem
Chemical SciencesPhysical Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 20 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 60 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf