Growth signals employ CGGBP1 to suppress transcription of Alu-SINEs
2016 (English)In: Cell Cycle, ISSN 1538-4101, E-ISSN 1551-4005, Vol. 15, no 12, 1558-1571 p.Article in journal (Refereed) Published
CGGBP1 (CGG triplet repeat-binding protein 1) regulates cell proliferation, stress response,cytokinesis, telomeric integrity and transcription. It could affect these processes by modulatingtarget gene expression under different conditions. Identification of CGGBP1-target genes andtheir regulation could reveal how a transcription regulator affects such diverse cellular processes.Here we describe the mechanisms of differential gene expression regulation by CGGBP1 inquiescent or growing cells. By studying global gene expression patterns and genome-wide DNAbindingpatterns of CGGBP1, we show that a possible mechanism through which it affects theexpression of RNA Pol II-transcribed genes in trans depends on Alu RNA. We also show that itregulates Alu transcription in cis by binding to Alu promoter. Our results also indicate thatpotential phosphorylation of CGGBP1 upon growth stimulation facilitates its nuclear retention,Alu-binding and dislodging of RNA Pol III therefrom. These findings provide insights into howAlu transcription is regulated in response to growth signals.
Place, publisher, year, edition, pages
2016. Vol. 15, no 12, 1558-1571 p.
Alu-SINEs; CGGBP1; ChIP-seq; growth signals; RNA Pol III; transcription; tyrosine phosphorylation
Research subject Bioinformatics; Biology
IdentifiersURN: urn:nbn:se:uu:diva-230959DOI: 10.4161/15384101.2014.967094ISI: 000379743800011PubMedID: 25483050OAI: oai:DiVA.org:uu-230959DiVA: diva2:742480
FunderSwedish Cancer SocietySwedish Research Council