Change search
ReferencesLink to record
Permanent link

Direct link
Neutrophils from vasculitis patients exhibit an increased propensity for activation by anti-neutrophil cytoplasmic antibodies
Lund University, Sweden .
Lund University, Sweden .
Linköping University, Department of Medical and Health Sciences, Division of Drug Research. Linköping University, Faculty of Health Sciences.
Lund University, Sweden .
Show others and affiliations
2014 (English)In: Clinical and Experimental Immunology, ISSN 0009-9104, E-ISSN 1365-2249, Vol. 176, no 3, 363-372 p.Article in journal (Refereed) Published
Abstract [en]

Anti-neutrophil cytoplasmic antibodies (ANCA) are thought to be pathogenic in ANCA-associated vasculitis (AAV) by stimulating polymorphonuclear leucocytes (PMNs) to degranulate and produce reactive oxygen species (ROS). The aim of this study was to investigate if PMNs from AAV patients are stimulated more readily by ANCA compared with PMNs from healthy controls (HCs). Differences in ANCA characteristics that can account for different stimulation potential were also studied. PMNs from five AAV patients and five HCs were stimulated with 10 different immunoglobulins (Ig)Gs, purified from PR3-ANCA-positive patients, and ROS production, degranulation and neutrophil extracellular trap (NET) formation was measured. ANCA levels, affinity and clinical data of the AAV donors were recorded. The results show that PMNs from AAV patients produce more intracellular ROS (P=0 center dot 019), but degranulate to a similar extent as PMNs from HCs. ROS production correlated with NET formation. Factors that may influence the ability of ANCA to activate PMNs include affinity and specificity for N-terminal epitopes. In conclusion, our results indicate that PMNs from AAV patients in remission behave quite similarly to HC PMNs, with the exception of a greater intracellular ROS production. This could contribute to more extensive NET formation and thus an increased exposure of the ANCA autoantigens to the immune system.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2014. Vol. 176, no 3, 363-372 p.
Keyword [en]
ANCA-associated vasculitis; autoimmunity; degranulation; reactive oxygen species
National Category
Clinical Medicine
URN: urn:nbn:se:liu:diva-109138DOI: 10.1111/cei.12301ISI: 000337516700008PubMedID: 24666336OAI: diva2:737514
Available from: 2014-08-13 Created: 2014-08-11 Last updated: 2014-11-04Bibliographically approved

Open Access in DiVA

Neutrophils from vasculitis patients exhibit an increased propensity for activation by anti-neutrophil cytoplasmic antibodies(1734 kB)210 downloads
File information
File name FULLTEXT01.pdfFile size 1734 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Söderberg, DanielSegelmark, Mårten
By organisation
Division of Drug ResearchFaculty of Health SciencesDepartment of Nephrology
In the same journal
Clinical and Experimental Immunology
Clinical Medicine

Search outside of DiVA

GoogleGoogle Scholar
Total: 210 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 74 hits
ReferencesLink to record
Permanent link

Direct link