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Organic phosphorus speciation in environmental samples: Method development and applications
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC.
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis investigates the development of new methodology for the identification and quantification of organic phosphorus compounds in environmental samples.

Phosphorus is a vital element for primary production and one of the factors contributing to eutrophication. Eutrophication of aquatic systems leads to algal blooms, changes in ecological balance and deteriorating water quality. Difficulties in studying organic phosphorus stem from the fact that organic phosphorus is present in the environment in a variety of forms and each form may have different degradation and turnover time, having very different effects on eutrophication.

New methods for the quantification of phosphorus derived from three groups of organic phosphorus compounds were developed. For the determination of phosphorus derived from DNA and phospholipids selective extraction was combined with digestion and colorimetric determination of the extracted phosphate. For quantification of inositol phosphates high performance liquid chromatography was coupled with tandem mass spectrometry using electrospray ionization.  

The methods were applied to studying the distribution of these compounds in a small catchment and in the case of DNA-P and phospholipid-P, the degradation of the fractions in lake sediments. The studies showed that phosphorus bound to DNA, phospholipids and inositol phosphates constitute a sizeable part of the total phosphorus in different environmental samples. The phospholipid-P fraction was the smallest one, accounting for, on average, only a few percent of the total phosphorus in the sample. Inositol phosphates were most prevalent in the soils, with inositol hexakisphosphate accounting for over 10% of the total phosphorus content. The highest content of DNA-P was found in sediments and it was shown that DNA-P degrades more rapidly than phospholipid-P and therefore plays a more critical role in internal loading.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2014. , 57 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1156
Keyword [en]
Organic phosphorus, DNA, phospholipids, inositol phosphates, sediment, soil, extraction, liquid chromatography (LC), mass spectrometry (MS)
National Category
Analytical Chemistry
Research subject
Chemistry with specialization in Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-228734ISBN: 978-91-554-8981-6 (print)OAI: oai:DiVA.org:uu-228734DiVA: diva2:734746
Public defence
2014-09-08, B41, BMC, Husargatan 3, Uppsala, 09:15 (English)
Opponent
Supervisors
Available from: 2014-08-18 Created: 2014-07-21 Last updated: 2014-09-08
List of papers
1. Extraction and quantification of phosphorus derived from DNA and lipids in environmental samples
Open this publication in new window or tab >>Extraction and quantification of phosphorus derived from DNA and lipids in environmental samples
2013 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 115, 336-341 p.Article in journal (Refereed) Published
Abstract [en]

 Understanding the flux and turnover of phosphorus (P) in the environment is important due to the key role P plays in eutrophication and in the ambition to find cost-effective measures to mitigate it. Orthophosphate diesters, including DNA and phospholipids (PLs), represent a potentially degradable P pool that could support future primary production and eutrophication. In this study, extraction techniques were optimized and combined with colorimetric determination of extracted P to provide a selective quantification method for DNA-P and PL-P in agricultural soil, sediment and composted manure. The proposed method is rapid and reproducible with an RSD of <10%. Recovery, evaluated by spiking the sample matrices with DNA and PL standards, was over 95% for both DNA and PLs. The method can be used for the determination of the pool size of the two organic P fractions. Results show that DNA-P comprises 3.0% by weight of the total P (TP) content in the studied soil, 10.4% in the sediment and 8.4% in the compost samples. The values for PL-P are 0.5%, 6.0% and 1.7% for soil, sediment and compost, respectively.

Keyword
Organic phosphorus, Extraction, Soil, Sediment, Compost
National Category
Chemical Sciences
Identifiers
urn:nbn:se:uu:diva-201968 (URN)10.1016/j.talanta.2013.05.042 (DOI)000328095600047 ()
Available from: 2013-06-18 Created: 2013-06-18 Last updated: 2017-12-06Bibliographically approved
2. Turnover of DNA-P and phospholipid-P in lake sediments
Open this publication in new window or tab >>Turnover of DNA-P and phospholipid-P in lake sediments
2014 (English)In: Biogeochemistry, ISSN 0168-2563, E-ISSN 1573-515X, Vol. 119, no 1-3, 361-370 p.Article in journal (Refereed) Published
Abstract [en]

Identifying and quantifying the forms of phosphorus (P) in lake sediments is a prerequisite for understanding lake trophic status and possible exports of P downstream. Organic P is one of the most important P forms found in the sediment, where orthophosphate diesters, including DNA and phospholipids, represent a degradable P pool that can support primary production and eutrophication. In this study, sediment cores from the eutrophic Lake Erken and the oligotrophic Lake Ånnsjön, both in steady state regarding long-term P input revealed trends in the degradation of DNA-P and PL-P with sediment depth. Comparisons were performed based on the differentiation of essentially permanent or recalcitrant P and temporary, potentially mobile P for the respective fractions. The temporary P pool was defined as the part of the total P pool calculated for values higher than the level at which the measured P concentration converged to a constant value and the recalcitrant pool was defined as the difference between the total and the temporary. The temporary diester-P pool comprised over 20 % of the total temporary P in Lake Erken and around 4 % in Lake Ånnsjön. The decrease in P concentrations with depth was more rapid for DNA-P compared to PL-P in both lakes, suggesting that DNA-P has a more prominent role in internal loading. The study shows that P mobilization potential can be different for different P fractions, which is important when assessing their contribution to internal loading of P within an aquatic system.

Keyword
Phosphorus turnover; Sediment; DNA; Phospholipids; Erken; Ånnsjön
National Category
Earth and Related Environmental Sciences Chemical Sciences
Identifiers
urn:nbn:se:uu:diva-224699 (URN)10.1007/s10533-014-9972-3 (DOI)000336028400023 ()
Available from: 2014-05-19 Created: 2014-05-19 Last updated: 2017-12-05Bibliographically approved
3. Speciation of inositol phosphates in lake sediments by ion-exchange chromatography coupled with mass spectrometry, ICP-AES and 31P NMR spectroscopy
Open this publication in new window or tab >>Speciation of inositol phosphates in lake sediments by ion-exchange chromatography coupled with mass spectrometry, ICP-AES and 31P NMR spectroscopy
Show others...
(English)Manuscript (preprint) (Other academic)
Keyword
Organic phosphorus, inositol phosphate, sediment, ion chromatography, HPLC-MS
National Category
Analytical Chemistry
Research subject
Chemistry with specialization in Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-228191 (URN)
Available from: 2014-07-21 Created: 2014-07-07 Last updated: 2014-09-08
4. Organic phosphorus speciation in a small catchment: sinks and sources
Open this publication in new window or tab >>Organic phosphorus speciation in a small catchment: sinks and sources
(English)Manuscript (preprint) (Other academic)
Keyword
organic phosphorus, DNA, phospholipid, inositol phosphate, soil, sediment, turnover
National Category
Analytical Chemistry Environmental Sciences related to Agriculture and Land-use Environmental Sciences
Research subject
Chemistry with specialization in Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-228193 (URN)
Available from: 2014-07-21 Created: 2014-07-07 Last updated: 2014-09-08

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