Quantitative characterization of articular cartilage using Mueller matrix imaging and multiphoton microscopy
2011 (English)In: Journal of Biomedical Optics, ISSN 1083-3668, E-ISSN 1560-2281, Vol. 16, no 11, 116002-1 p.Article in journal (Refereed) Published
The collagen meshwork in articular cartilage of chicken knee is characterized using Mueller matrix imaging and multiphoton microscopy. Direction and degree of dispersion of the collagen fibers in the superficial layer are found using a Fourier transform image-analysis technique of the second-harmonic generated image. Mueller matrix images are used to acquire structural data from the intermediate layer of articular cartilage where the collagen fibers are too small to be resolved by optical microscopy, providing a powerful multimodal measurement technique. Furthermore, we show that Mueller matrix imaging provides more information about the tissue compared to standard polarization microscopy. The combination of these techniques can find use in improved diagnosis of diseases in articular cartilage, improved histopathology, and additional information for accurate biomechanical modeling of cartilage.
Place, publisher, year, edition, pages
SPIE - International Society for Optical Engineering, 2011. Vol. 16, no 11, 116002-1 p.
IdentifiersURN: urn:nbn:no:ntnu:diva-25338DOI: 10.1117/1.3643721OAI: oai:DiVA.org:ntnu-25338DiVA: diva2:734576
Copyright 2014 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic electronic or print reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited2014-07-182014-07-182014-09-23Bibliographically approved