Change search
ReferencesLink to record
Permanent link

Direct link
Regulation of alginate biosynthesis in Azotobacter vinelandii
Norwegian University of Science and Technology, Faculty of Natural Sciences and Technology, Department of Biotechnology.
2014 (English)MasteroppgaveStudent thesis
Abstract [en]

Alginate is a linear exopolysaccharide composed of various amount of (1-4) linked β-D mannuronic acid and its C5 epimer α-L-guluronic acid. Alginate is synthesized by two bacterial genera Azotobacter and Pseudomonas. Azotobacter vinelandii is a gram negative bacterium that can fix atmospheric nitrogen and form desiccation resistant cyst. Transcriptional activation of algD gene is a main point in regulation of alginate biosynthesis pathway in A.vinelandii. In A.vinelandii alginate biosynthesis gene cluster is organized in transcriptional units of algD promoter. AlgR, AlgB and AmrZ are the major regulators required by algD promoter for its expression. Alternative sigma factor σ22 encoded by algU directly controls the algD promoter and its expression. In this study, a vector expressing AlgW in A.vinelandii was constructed. Also, the plasmids for measuring algD expression and algC expression using mcherry as a reporter gene were constructed. Alginate production and algD gene expression was compared in strains lacking algW or algB with wildtype strain overproducing these proteins. Also, alginate was assayed enzymatically. Based on results it was found that algB controls algD not only in Pseudomonas species but also in Azotobacter. The study of effect of succinate on growth and alginate revealed that wildtype A.vinelandii has maximum growth in the medium supplemented with succinate.

Place, publisher, year, edition, pages
Institutt for bioteknologi , 2014. , 62 p.
URN: urn:nbn:no:ntnu:diva-25118Local ID: ntnudaim:8747OAI: diva2:731567
Available from: 2014-07-01 Created: 2014-07-01 Last updated: 2014-07-01Bibliographically approved

Open Access in DiVA

fulltext(1651 kB)638 downloads
File information
File name FULLTEXT01.pdfFile size 1651 kBChecksum SHA-512
Type fulltextMimetype application/pdf
cover(184 kB)14 downloads
File information
File name COVER01.pdfFile size 184 kBChecksum SHA-512
Type coverMimetype application/pdf

By organisation
Department of Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
Total: 638 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 114 hits
ReferencesLink to record
Permanent link

Direct link