Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A dynamic interplay of nucleosome and Msn2 binding regulates kinetics of gene activation and repression following stress
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
Show others and affiliations
2014 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 42, no 9, 5468-5482 p.Article in journal (Refereed) Published
Abstract [en]

The transcription factor Msn2 mediates a significant proportion of the environmental stress response, in which a common cohort of genes changes expression in a stereotypic fashion upon exposure to any of a wide variety of stresses. We have applied genome-wide chromatin immunoprecipitation and nucleosome profiling to determine where Msn2 binds under stressful conditions and how that binding affects, and is affected by, nucleosome positioning. We concurrently determined the effect of Msn2 activity on gene expression following stress and demonstrated that Msn2 stimulates both activation and repression. We found that some genes responded to both intermittent and continuous Msn2 nuclear occupancy while others responded only to continuous occupancy. Finally, these studies document a dynamic interplay between nucleosomes and Msn2 such that nucleosomes can restrict access of Msn2 to its canonical binding sites while Msn2 can promote reposition, expulsion and recruitment of nucleosomes to alter gene expression. This interplay may allow the cell to discriminate between different types of stress signaling.

Place, publisher, year, edition, pages
2014. Vol. 42, no 9, 5468-5482 p.
National Category
Medical Genetics
Identifiers
URN: urn:nbn:se:umu:diva-90879DOI: 10.1093/nar/gku176ISI: 000336495400015OAI: oai:DiVA.org:umu-90879DiVA: diva2:731504
Available from: 2014-07-01 Created: 2014-07-01 Last updated: 2017-12-05Bibliographically approved

Open Access in DiVA

fulltext(15798 kB)109 downloads
File information
File name FULLTEXT01.pdfFile size 15798 kBChecksum SHA-512
c81a976fa26f92a6fd76ca3d023d3c45ad91f0c8c7b4caef224325be510a13055d3f0dd31d5a1d4e74b29d9f17aa6da7643e476462b4892c3a4385ddbf8fd7dc
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Elfving, NilsBjörklund, Stefan
By organisation
Department of Medical Biochemistry and Biophysics
In the same journal
Nucleic Acids Research
Medical Genetics

Search outside of DiVA

GoogleGoogle Scholar
Total: 109 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 172 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf