Evaluation of ELISA and rapid test for the analysis of fecal Calprotectin
Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Background Calprotectin is a protein found in the cytoplasm of neutrophile granulocytes. In the course of inflammatory bowel disease (IBD), calprotectin is released during chronic inflammation in the gut. Activation of neutrophils during the inflammation is followed by activation and secretion of pro-inflammatory molecules such as calprotectin. Calprotectin is stable in stool up to 7 days and can therefore be used as a non-invasive marker for diagnosis, treatment and measurement of the disease activity in patients with IBD. The most common method for analysis of calprotectin concentration is ELISA. This method is time-consuming and many manufactures have therefore developed rapid tests as a faster alternative for quantification of calprotectin in stool.
Aim The aim of the study was to evaluate one ELISA and one rapid test from the same manufacture compare the data with the existing ELISA-method used in the laboratory for routine analysis.
Methods A rapid test (CalFast) and an ELISA method (CalPrest) from Eurospital, were used for analysis of calprotectin in stool. These two methods were compared with known concentrations of calprotectin obtained by the ELISA method from Bühlmann used in the routine work.
Results The results showed poor correlation between the rapid test and the ELISA method. Furthermore, the comparison between the two ELISA-methods showed a poor correlation.
Conclusion Evaluation of the two new methods showed poor correlation with the existing ELISA method from Bühlmann. Evaluation of the rapid test did not show any correlation with the two ELISA methods and the data cannot be trusted. It is difficult to conclude which of the two ELISA methods gives accurate results due to the absence of an international standard.
Place, publisher, year, edition, pages
IBD, Calprotectin, Non-invasive marker, ELISA, rapid test
Biomedical Laboratory Science/Technology
IdentifiersURN: urn:nbn:se:uu:diva-227234OAI: oai:DiVA.org:uu-227234DiVA: diva2:728716
Biomedical Laboratory Science Programme
Mandic- Havelka, Aleksandra
Stavreus-Evers, Anneli, Docent