Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
DNA Methylation Changes Separate Allergic Patients from Healthy Controls and May Reflect Altered CD4(+) T-Cell Population Structure
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Health Sciences.
Show others and affiliations
2014 (English)In: PLoS Genetics, ISSN 1553-7390, E-ISSN 1553-7404, Vol. 10, no 1, e1004059- p.Article in journal (Refereed) Published
Abstract [en]

Altered DNA methylation patterns in CD4(+) T-cells indicate the importance of epigenetic mechanisms in inflammatory diseases. However, the identification of these alterations is complicated by the heterogeneity of most inflammatory diseases. Seasonal allergic rhinitis (SAR) is an optimal disease model for the study of DNA methylation because of its welldefined phenotype and etiology. We generated genome-wide DNA methylation (N-patients = 8, N-controls = 8) and gene expression (N-patients = 9, N-controls = 10) profiles of CD4(+) T-cells from SAR patients and healthy controls using Illuminas HumanMethylation450 and HT-12 microarrays, respectively. DNA methylation profiles clearly and robustly distinguished SAR patients from controls, during and outside the pollen season. In agreement with previously published studies, gene expression profiles of the same samples failed to separate patients and controls. Separation by methylation (N-patients = 12, N-controls = 12), but not by gene expression (N-patients = 21, N-controls = 21) was also observed in an in vitro model system in which purified PBMCs from patients and healthy controls were challenged with allergen. We observed changes in the proportions of memory T-cell populations between patients (N-patients = 35) and controls (N-controls = 12), which could explain the observed difference in DNA methylation. Our data highlight the potential of epigenomics in the stratification of immune disease and represents the first successful molecular classification of SAR using CD4(+) T cells.

Place, publisher, year, edition, pages
Public Library of Science , 2014. Vol. 10, no 1, e1004059- p.
National Category
Clinical Medicine Basic Medicine
Identifiers
URN: urn:nbn:se:liu:diva-107871DOI: 10.1371/journal.pgen.1004059ISI: 000336525000030OAI: oai:DiVA.org:liu-107871DiVA: diva2:727638
Available from: 2014-06-23 Created: 2014-06-23 Last updated: 2017-12-05

Open Access in DiVA

fulltext(1349 kB)112 downloads
File information
File name FULLTEXT01.pdfFile size 1349 kBChecksum SHA-512
a2d581f83b7972624d4d49bbddbda8f86f337e1c9110499af0c52f67ba95935829305517a2e6848a1f7df64183be430ba7027fe3e2589367b3494ecab925877d
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Nestor, ColmBarrenäs, FredrikWang, HuiZhang, HuanBruhn, SörenGustafsson, MikaBenson, Mikael

Search in DiVA

By author/editor
Nestor, ColmBarrenäs, FredrikWang, HuiZhang, HuanBruhn, SörenGustafsson, MikaBenson, Mikael
By organisation
Division of Clinical SciencesFaculty of Health SciencesAllergy CenterDepartment of Paediatrics in Linköping
In the same journal
PLoS Genetics
Clinical MedicineBasic Medicine

Search outside of DiVA

GoogleGoogle Scholar
Total: 112 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 171 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf