A Real Time Immunoassay in Alumina Membranes
2014 (English)In: SENSORS, 2014 IEEE, IEEE conference proceedings, 2014, 1760-1763 p.Conference paper, Abstract (Refereed)
To date, photonic biosensing with porous membranes has produced slow responses and long sensing times, due to the narrow (less than 100 nm) closed end pores of the membranes used. Recently, polarimetry was used to demonstrate analyte flow through, and real time biosensing in, free-standing porous alumina membranes. Here, we demonstrate how an improved functionalization technology, has for the first time enabled a real-time immunoassay within a porous membrane with a total assay time below one hour. With the new approach, we show a noise floor for individual biosensing measurements of 3.7 ng/ml (25 pM), and a bulk refractive index detection limit of 5×10-6 RIU, with a standard deviation of less than 5%. The membranes, with their 200 nm pore diameter enabling targeted delivering of analytes to bioreceptors immobilized on the pore walls, therefore provide a route towards rapid and low cost real-time opto-fluidic biosensors for small sample volumes.
Place, publisher, year, edition, pages
IEEE conference proceedings, 2014. 1760-1763 p.
Biosensor, porous alumina
Other Electrical Engineering, Electronic Engineering, Information Engineering
Research subject Electrical Engineering
IdentifiersURN: urn:nbn:se:kth:diva-146997DOI: 10.1109/ICSENS.2014.6985365OAI: oai:DiVA.org:kth-146997DiVA: diva2:727282
IEEE Sensors 2014,Valencia, Spain, November 2-5, 2014
FunderEU, FP7, Seventh Framework Programme, 257401
QC 201502032014-06-192014-06-192015-08-12Bibliographically approved