Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Infected or not: are PCR-positive oropharyngeal swabs indicative of low pathogenic influenza A virus infection in the respiratory tract of Mallard Anas platyrhynchos?
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science. (Zoonotic Ecology and Epidemiology / EEMiS)ORCID iD: 0000-0002-5629-0196
Erasmus Medical Centre, The Netherlands.
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science. (EEMiS)ORCID iD: 0000-0002-1152-4235
Erasmus Medical Centre, The Netherlands.
2014 (English)In: Veterinary research (Print), ISSN 0928-4249, E-ISSN 1297-9716, Vol. 45, Article ID: 53- p.Article in journal (Refereed) Published
Abstract [en]

Detection of influenza virus in oropharyngeal swabs collected during wild bird surveillance is assumed to representrespiratory infection, although intestine is the main site of infection. We tested this assumption by histologicalexamination of the respiratory tract of wild Mallards with virus-positive oropharyngeal swabs. Thirty-two of 125Mallards tested had viral-RNA positive oropharyngeal swabs. The respiratory tracts of four Mallards with the mostvirus were examined in detail by immunohistochemistry. None had detectable virus antigen in the respiratory tract,suggesting it was not infected. An alternative explanation is that the oropharynx was contaminated with virusthrough feeding in surface water or through preening.

Place, publisher, year, edition, pages
2014. Vol. 45, Article ID: 53- p.
National Category
Microbiology
Research subject
Natural Science, Zoonotic Ecology
Identifiers
URN: urn:nbn:se:lnu:diva-34673DOI: 10.1186/1297-9716-45-53ISI: 000336928100001Scopus ID: 2-s2.0-84902380893OAI: oai:DiVA.org:lnu-34673DiVA: diva2:721672
Available from: 2014-06-04 Created: 2014-06-04 Last updated: 2017-12-05Bibliographically approved
In thesis
1. Viruses on the wing: evolution and dynamics of influenza A virus in the Mallard reservoir
Open this publication in new window or tab >>Viruses on the wing: evolution and dynamics of influenza A virus in the Mallard reservoir
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis explores the evolution of avian influenza A viruses (IAV), as well as host-pathogen interactions between these viruses and their main reservoir host, the Mallard (Anas platyrhynchos). IAV is a genetically diverse, multi-host virus and wild birds, particularly dabbling ducks, are the natural reservoir. At our study site, up to 30% of migratory Mallards are infected with IAV during an autumn season, and host a large number of virus subtypes. IAV diversity is driven by two main mechanisms: mutation, driving genetic drift; and reassortment following co-infection, resulting in genetic shift.

 

Reassortment is pervasive within an autumn season, both across multiple subtypes and within a single subtype. It is a key genetic feature in long-term maintenance of common subtypes, as it allows for independent lineage turn-over, generating novel genetic constellations. I hypothesize that the decoupling of successful constellations and generation of novel annual constellations enables viruses to escape herd immunity; these genetic changes must confer antigenic change for the process to be favourable. Indeed, in an experiment utilizing vaccines, circulating viruses escaped homosubtypic immunity, resulting in the proliferation of infections with the same subtype as the vaccine. While the host plays an important role in shaping IAV evolutionary genetics, one must consider that Mallards are infected with a multitude of other microorganisms. Here, Mallards were infected with IAV, gamma coronaviruses, and avian paramyxovirus type 1 simultaneously, and we found a putative synergistic interaction between IAV and gamma coronaviruses.

 

Mallards occupy the interface between humans, poultry, and wild birds, and are the reservoir of IAV diversity. New incursions of highly pathogenic H5 viruses to both Europe and North America reaffirms the role of wild birds, particularly waterfowl, in diffusion of viruses spatially. Using European low pathogenic viruses and Mallard model, this thesis contributes to aspects of epidemiology, ecology, and evolutionary dynamics of waterfowl viruses, particularly IAV

Place, publisher, year, edition, pages
Växjö: Linnaeus University Press, 2015
Series
Linnaeus University Dissertations, 217/2015
Keyword
Co-infections, Ecology, Epidemiology, Evolution, Host-Parasite Interactions, Immunity, Influenza A Virus, Mallard, Phylogeny, Virology, Waterfowl, Wild Birds, Wildlife Disease
National Category
Ecology
Research subject
Natural Science, Zoonotic Ecology
Identifiers
urn:nbn:se:lnu:diva-41431 (URN)978-91-87925-56-6 (ISBN)
Public defence
2015-05-08, Fullriggaren, Landgången 4, Kalmar, 09:30 (English)
Opponent
Supervisors
Available from: 2015-03-27 Created: 2015-03-27 Last updated: 2015-12-04Bibliographically approved

Open Access in DiVA

Wille_2014_VetRes_IHC(600 kB)45 downloads
File information
File name FULLTEXT01.pdfFile size 600 kBChecksum SHA-512
83a2d392c9108bbcdab0cda4b66f6248d9b565a7ee9798a108c0d44d27eb800d7ff61d3eb8aa8f988bf00e2a7848a6d573b1d2c13bb03caeac71719395d17aec
Type fulltextMimetype application/pdf

Other links

Publisher's full textScopusfulltext

Search in DiVA

By author/editor
Wille, MichelleWaldenström, Jonas
By organisation
Department of Biology and Environmental Science
In the same journal
Veterinary research (Print)
Microbiology

Search outside of DiVA

GoogleGoogle Scholar
Total: 45 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 119 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf