Change search
ReferencesLink to record
Permanent link

Direct link
Increased paired box transcription factor 8 has a survival function in Glioma
Umeå University, Faculty of Medicine, Department of Pharmacology and Clinical Neuroscience, Clinical Neuroscience.
Show others and affiliations
2014 (English)In: BMC Cancer, ISSN 1471-2407, Vol. 14, 159- p.Article in journal (Refereed) Published
Abstract [en]


The molecular basis to overcome therapeutic resistance to treat glioblastoma remains unclear. The anti-apoptotic b cell lymphoma 2 (BCL2) gene is associated with treatment resistance, and is transactivated by the paired box transcription factor 8 (PAX8). In earlier studies, we demonstrated that increased PAX8 expression in glioma cell lines was associated with the expression of telomerase. In this current study, we more extensively explored a role for PAX8 in gliomagenesis.


PAX8 expression was measured in 156 gliomas including telomerase-negative tumours, those with the alternative lengthening of telomeres (ALT) mechanism or with a non-defined telomere maintenance mechanism (NDTMM), using immunohistochemistry and quantitative PCR. We also tested the affect of PAX8 knockdown using siRNA in cell lines on cell survival and BCL2 expression.


Seventy-two percent of glioblastomas were PAX8-positive (80% telomerase, 73% NDTMM, and 44% ALT). The majority of the low-grade gliomas and normal brain cells were PAX8-negative. The suppression of PAX8 was associated with a reduction in both cell growth and BCL2, suggesting that a reduction in PAX8 expression would sensitise tumours to cell death.


PAX8 is increased in the majority of glioblastomas and promoted cell survival. Because PAX8 is absent in normal brain tissue, it may be a promising therapeutic target pathway for treating aggressive gliomas.

Place, publisher, year, edition, pages
2014. Vol. 14, 159- p.
Keyword [en]
PAX8, Glioblastoma, Glioma, Telomere maintenance mechanism, Telomerase, ALT, BCL2, Cell survival
National Category
Medical and Health Sciences
URN: urn:nbn:se:umu:diva-88287DOI: 10.1186/1471-2407-14-159ISI: 000333420600003PubMedID: 24602166OAI: diva2:715614
Available from: 2014-05-05 Created: 2014-04-29 Last updated: 2014-05-05Bibliographically approved

Open Access in DiVA

Increased paired box transcription factor 8 has a survival function in Glioma(2259 kB)272 downloads
File information
File name FULLTEXT01.pdfFile size 2259 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Olivecrona, Magnus
By organisation
Clinical Neuroscience
In the same journal
BMC Cancer
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 272 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 33 hits
ReferencesLink to record
Permanent link

Direct link