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Heterologous overexpression of a monotopic glucosyltransferase (MGS) induces fatty acid remodeling in Escherichia coli membranes
The Arrhenius Laboratories for Natural Sciences, Department of Biochemistry and Biophysics, Stockholm University, Sweden.
The Arrhenius Laboratories for Natural Sciences, Department of Biochemistry and Biophysics, Stockholm University, Sweden.
The Arrhenius Laboratories for Natural Sciences, Department of Biochemistry and Biophysics, Stockholm University, Sweden.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
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2014 (English)In: Biochimica et Biophysica Acta - Biomembranes, ISSN 0005-2736, E-ISSN 1879-2642, Vol. 1838, no 7, 1862-1870 p.Article in journal (Refereed) Published
Abstract [en]

The membrane protein monoglucosyldiacylglycerol synthase (MGS) from Acholeplasma laidlawii is responsible for the creation of intracellularmembraneswhen overexpressed in Escherichia coli (E. coli). The present study investigates time dependent changes in composition and properties of E. coli membranes during 22 h of MGS induction. The lipid/protein ratio increased by 38% in MGS-expressing cells compared to control cells. Time-dependent screening of lipids during this period indicated differences in fatty acid modeling. (1) Unsaturation levels remained constant for MGS cells (~62%) but significantly decreased in control cells (from 61% to 36%). (2) Cyclopropanated fatty acid content was lower in MGS producing cells while control cells had an increased cyclopropanation activity. Among all lipids, phosphatidylethanolamine (PE)was detected to be themost affected species in terms of cyclopropanation. Higher levels of unsaturation, lowered cyclopropanation levels and decreased transcription of the gene for cyclopropane fatty acid synthase (CFA) all indicate the tendency of the MGS protein to force E. coli membranes to alter its usual fatty acid composition.

Place, publisher, year, edition, pages
2014. Vol. 1838, no 7, 1862-1870 p.
Keyword [en]
Lipid–protein interaction, Fatty acid modeling, Cyclopropanation, Membrane fluidity, Monotopic membrane protein, Intracellular membrane
National Category
Chemical Sciences Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-223782DOI: 10.1016/j.bbamem.2014.04.001ISI: 000336695300020OAI: oai:DiVA.org:uu-223782DiVA: diva2:714106
Available from: 2014-04-25 Created: 2014-04-25 Last updated: 2017-12-05Bibliographically approved

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