Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Stable corneal regeneration four years after implantation of a cell-free recombinant human collagen scaffold
Linköping University, Department of Clinical and Experimental Medicine, Division of Neuroscience. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Anaesthetics, Operations and Specialty Surgery Center, Department of Ophthalmology in Linköping.
Linköping University, Department of Clinical and Experimental Medicine, Division of Neuroscience. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Anaesthetics, Operations and Specialty Surgery Center, Department of Ophthalmology in Linköping.
Maisonneuve Rosemont Hospital, Montreal, Canada .
Östergötlands Läns Landsting, Anaesthetics, Operations and Specialty Surgery Center, Department of Ophthalmology in Linköping. Ottawa Hospital Research Institute, Canada.
Show others and affiliations
2014 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 35, no 8, 2420-2427 p.Article in journal (Refereed) Published
Abstract [en]

We developed cell-free implants, comprising carbodiimide crosslinked recombinant human collagen (RHC), to enable corneal regeneration by endogenous cell recruitment, to address the worldwide shortage of donor corneas. Patients were grafted with RHC implants. Over four years, the regenerated neo-corneas were stably integrated without rejection, without the long immunosuppression regime needed by donor cornea patients. There was no recruitment of inflammatory dendritic cells into the implant area, whereas, even with immunosuppression, donor cornea recipients showed dendritic cell migration into the central cornea and a rejection episode was observed. Regeneration as evidenced by continued nerve and stromal cell repopulation occurred over the four years to approximate the micro-architecture of healthy corneas. Histopathology of a regenerated, clear cornea from a regrafted patient showed normal corneal architecture. Donor human cornea grafted eyes had abnormally tortuous nerves and stromal cell death was found. Implanted patients had a 4-year average corrected visual acuity of 20/54 and gained more than 5 Snellen lines of vision on an eye chart. The visual acuity can be improved with more robust materials for better shape retention. Nevertheless, these RHC implants can achieve stable regeneration and therefore, represent a potentially safe alternative to donor organ transplantation.

Place, publisher, year, edition, pages
Elsevier, 2014. Vol. 35, no 8, 2420-2427 p.
Keyword [en]
Cornea; Collagen; Transplantation; Recombinant protein
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-105571DOI: 10.1016/j.biomaterials.2013.11.079ISI: 000331679200003PubMedID: 24374070OAI: oai:DiVA.org:liu-105571DiVA: diva2:708867
Available from: 2014-03-31 Created: 2014-03-27 Last updated: 2017-12-05Bibliographically approved

Open Access in DiVA

fulltext(2492 kB)137 downloads
File information
File name FULLTEXT01.pdfFile size 2492 kBChecksum SHA-512
b58037857d4975e0a2532a85f1712037d51b858c2b54f3bca4d34379f0816522fb1d189cd660513284fd8f97ade0531902efbdd6f2b82fb533a5d7e16052798e
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Fagerholm, PerLagali, NeilGriffith, May

Search in DiVA

By author/editor
Fagerholm, PerLagali, NeilGriffith, May
By organisation
Division of NeuroscienceFaculty of Health SciencesDepartment of Ophthalmology in LinköpingDivision of Cell Biology
In the same journal
Biomaterials
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 137 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 903 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf