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Development of a label-free biosensor method for the identification of sticky compounds which disturb GPCR-assays
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2013 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

It is widely known that early estimates about the binding properties of drug candidates are important in the drug discovery process. Surface plasmon resonance (SPR) biosensors have become a standard tool for characterizing interactions between a great variety of biomolecules and it offers a unique opportunity to study binding activity.

The aim of this project was to develop a SPR based assay for pre-screening of low molecular weight (LMW) drug compounds, to enable filtering away disturbing compounds when interacting with drugs. The interaction between 47 LMW compounds and biological ligands were investigated using the instrument BiacoreTM, which is based on SPR-technology.

Place, publisher, year, edition, pages
2013. , 45 p.
Series
UPTEC X, 13025
Keyword [en]
Surface plasmon resonance (SPR), biosensor, Biacore, G-protein-coupled receptors (GPCRs), Low molecular weight (LMW) compounds, C-C chemokine receptor type 5 (CCR5), acid-sensing ion channel 1a (ASIC1a), Thrombin, Carbonic Anhydrase II (CA II), P38α MAP kinase.
National Category
Biomedical Laboratory Science/Technology Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-220645OAI: oai:DiVA.org:uu-220645DiVA: diva2:705969
External cooperation
GE Healthcare
Educational program
Molecular Biotechnology Engineering Programme
Supervisors
Examiners
Available from: 2014-03-19 Created: 2014-03-18 Last updated: 2014-03-19Bibliographically approved

Open Access in DiVA

Master Thesis, Examensrapport - Hamno Mohammed Kader(4344 kB)530 downloads
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Biomedical Laboratory Science/TechnologyBiochemistry and Molecular Biology

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CiteExportLink to record
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