Development of a label-free biosensor method for the identification of sticky compounds which disturb GPCR-assays
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
It is widely known that early estimates about the binding properties of drug candidates are important in the drug discovery process. Surface plasmon resonance (SPR) biosensors have become a standard tool for characterizing interactions between a great variety of biomolecules and it offers a unique opportunity to study binding activity.
The aim of this project was to develop a SPR based assay for pre-screening of low molecular weight (LMW) drug compounds, to enable filtering away disturbing compounds when interacting with drugs. The interaction between 47 LMW compounds and biological ligands were investigated using the instrument BiacoreTM, which is based on SPR-technology.
Place, publisher, year, edition, pages
2013. , 45 p.
UPTEC X, 13025
Surface plasmon resonance (SPR), biosensor, Biacore, G-protein-coupled receptors (GPCRs), Low molecular weight (LMW) compounds, C-C chemokine receptor type 5 (CCR5), acid-sensing ion channel 1a (ASIC1a), Thrombin, Carbonic Anhydrase II (CA II), P38α MAP kinase.
Biomedical Laboratory Science/Technology Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-220645OAI: oai:DiVA.org:uu-220645DiVA: diva2:705969
Molecular Biotechnology Engineering Programme
Pol, Ewa, PhD
Josefsson, Lars-Göran, PhD