Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Development and evaluation of procedures and reagents for extraction of proteins from dried blood spots for analysis using Proseek
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2014 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

A method for extraction of proteins from dried blood spots (DBS) for analysis using Proseek is developed and evaluated. DBS, as sample format, possesses a number of desirable advantages over for example plasma samples. These advantages include for example minimal patient invasiveness, sampling simplicity and non regulated sample transportation. Highly reproducible quantitative detection of 92 proteins is demonstrated from a 1.2 mm in diameter DBS disk. The DBS inter spot analysis precision (7% coefficient of variance) is comparable to plasma inter assay precision (6% coefficient of variance). Coefficient of variance is the ratio between standard deviation to mean value for the analysed replicates. Proseek analysis of DBS could possibly reveal a unique opportunity to examine health related issues in extremely premature infants hopefully resulting in increased survival rates in the future.

Place, publisher, year, edition, pages
2014. , 64 p.
Series
UPTEC X, 14 001
Keyword [en]
DBS, dried blood spots, PEA, Proseek, Proseek Multiplex, qPCR, Protein detection, Olink
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-219292OAI: oai:DiVA.org:uu-219292DiVA: diva2:699065
Educational program
Molecular Biotechnology Engineering Programme
Supervisors
Examiners
Available from: 2014-02-26 Created: 2014-02-26 Last updated: 2014-02-26Bibliographically approved

Open Access in DiVA

UPTEC X 14 001 Johan Björkesten(3573 kB)719 downloads
File information
File name FULLTEXT01.pdfFile size 3573 kBChecksum SHA-512
e3b203be2cb4573ff1ea236b5e3b5291c70a13c0a71b5c94ab49d6516a06c2746e9ce55009eb39f0c15289a7890cacc6851900177fa91f80719de90780de9d93
Type fulltextMimetype application/pdf

By organisation
Biology Education Centre
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 719 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 537 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf