Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
TGF-β (BETA) AND PERIOSTIN MODULATE EACH OTHER’S EXPRESSION IN BOTH BREAST STROMA AND TUMOR CELLS
University of Skövde, School of Life Sciences.
2013 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Breast cancer is the most common cancer in female population worldwide. In addition to mutations, the breast tumor microenvironment especially the tumor cell - stroma interactions through extracellular matrix components and multiple growth factors have been shown to promote tumor progression. Among those, increases in both TGF-β (transforming growth factor beta) activities and periostin expression were associated with tumor cell survival, proliferation and metastasis. TGF-β role in breast cancer progression including its ability to promote periostin expression has been extensively studied. In contrast, the role of periostin in cancer progression remains to be fully understood. Thus, the present study aimed to determine whether TGF-β and periostin have effect on each other’s expressions in breast tumor and stroma cells using in vitro cell models. Through Western blot analyses and ELISAs, the periostin and TGF-β expressions of both stroma and tumor cells were analyzed following TGF-β and periostin treatments, respectively. The results indicate that TGF-β treatments led to significant increase in periostin expression in fibroblasts (p<0.05). In addition, periostin was differentially expressed by human breast cancer cells following TGF-β1 treatment. The TGF-β activities involved activation of pSMAD2 in both L929 fibroblasts and MCF10A mammary cells. Taken together, all experimental data indicate that within the breast tumor TGF-β and periostin likely participate in a regulation loop. Whether this putative regulation loop is critical to metastasis remains to be determined. Should periostin play a critical role in breast cancer progression, it could become a specific target in the preventive and/or therapeutic development of breast cancer patients.

Place, publisher, year, edition, pages
2013. , 30 p.
Keyword [en]
TGF-β, Periostin, Breast Stroma, Breast Tumor, Tumor Microenvironment
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:his:diva-8893OAI: oai:DiVA.org:his-8893DiVA: diva2:699013
Subject / course
Molecular Biology
Educational program
Molekular Biology - Master's Programme
Presentation
2013-06-05, Skövde, 09:00 (English)
Supervisors
Examiners
Available from: 2014-03-03 Created: 2014-02-26 Last updated: 2014-03-03Bibliographically approved

Open Access in DiVA

USkovde_Master Degree Thesis (2013)_Anindita Das Burman(1231 kB)749 downloads
File information
File name FULLTEXT01.pdfFile size 1231 kBChecksum SHA-512
121e8ece53ad6b2d751afd1e7f615466ac8a3ca83e2985872c3d71e50807fc45f75b39efde534b52b56cd142d7a9b42017e29acb90837fae6bdba30f3c8a64bf
Type fulltextMimetype application/pdf

By organisation
School of Life Sciences
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 749 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 163 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf