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LIGATION-BASED MUTATION DETECTION AND RCA IN SURFACE UN-MODIFIED OSTE+ POLYMER MICROFLUIDIC CHAMBERS
KTH, School of Electrical Engineering (EES), Micro and Nanosystems.
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2013 (English)In: 17th IEEE International Conference on Solid-State Sensors, Actuators and Microsystems & EUROSENSORS XXVII (IEEE TRANSDUCERS 2013), IEEE conference proceedings, 2013, 357-360 p.Conference paper, Published paper (Refereed)
Abstract [en]

For the first time, we demonstrate DNA mutation detection in surface un-modified polymeric microfluidic chambers without suffering from bubble trapping or bubble formation. Microfluidic devices were manufactured in off-stoichiometry thiol-ene epoxy (OSTE+) polymer using an uncomplicated and rapid fabrication scheme. The device performance was compared to that of PMMA and PDMS devices. In OSTE+ devices, we were able to perform ligation-based mutation detection and rolling circle amplification (RCA) assays directly on the un-modified surface without suffering from bubble formation or enzyme inhibition during bio-operation at elevated temperatures. In contrast, PMMA, PDMS and COP microfluidic devices required specific surface treatment.

Place, publisher, year, edition, pages
IEEE conference proceedings, 2013. 357-360 p.
Keyword [en]
Epoxy, lab-on-a-chip, microfluidics, Molecular diagnostics, off-stoichiometry thiol-ene, OSTE+, polymer, RCA, rolling circle amplification
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:kth:diva-141505DOI: 10.1109/Transducers.2013.6626776Scopus ID: 2-s2.0-84891721243ISBN: 978-146735981-8 (print)OAI: oai:DiVA.org:kth-141505DiVA: diva2:697371
Conference
17th IEEE International Conference on Solid-State Sensors, Actuators and Microsystems & EUROSENSORS XXVII (IEEE TRANSDUCERS 2013), Barcelona, SPAIN, 16-20 Jun, 2013
Note

QC 20140218

Available from: 2014-02-18 Created: 2014-02-18 Last updated: 2015-06-03Bibliographically approved

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