LIGATION-BASED MUTATION DETECTION AND RCA IN SURFACE UN-MODIFIED OSTE+ POLYMER MICROFLUIDIC CHAMBERS
2013 (English)In: 17th IEEE International Conference on Solid-State Sensors, Actuators and Microsystems & EUROSENSORS XXVII (IEEE TRANSDUCERS 2013), IEEE conference proceedings, 2013, 357-360 p.Conference paper (Refereed)
For the first time, we demonstrate DNA mutation detection in surface un-modified polymeric microfluidic chambers without suffering from bubble trapping or bubble formation. Microfluidic devices were manufactured in off-stoichiometry thiol-ene epoxy (OSTE+) polymer using an uncomplicated and rapid fabrication scheme. The device performance was compared to that of PMMA and PDMS devices. In OSTE+ devices, we were able to perform ligation-based mutation detection and rolling circle amplification (RCA) assays directly on the un-modified surface without suffering from bubble formation or enzyme inhibition during bio-operation at elevated temperatures. In contrast, PMMA, PDMS and COP microfluidic devices required specific surface treatment.
Place, publisher, year, edition, pages
IEEE conference proceedings, 2013. 357-360 p.
Epoxy, lab-on-a-chip, microfluidics, Molecular diagnostics, off-stoichiometry thiol-ene, OSTE+, polymer, RCA, rolling circle amplification
Engineering and Technology
IdentifiersURN: urn:nbn:se:kth:diva-141505DOI: 10.1109/Transducers.2013.6626776ScopusID: 2-s2.0-84891721243ISBN: 978-146735981-8OAI: oai:DiVA.org:kth-141505DiVA: diva2:697371
17th IEEE International Conference on Solid-State Sensors, Actuators and Microsystems & EUROSENSORS XXVII (IEEE TRANSDUCERS 2013), Barcelona, SPAIN, 16-20 Jun, 2013
QC 201402182014-02-182014-02-182015-06-03Bibliographically approved